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Assay Detail

CHEMBL871564

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Activity against human GLUR2 flip expressed in HEK293 cells assessed as glutamate-stimulated calcium influx by FLIPR assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Glutamate receptor 2 (CHEMBL4016)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A novel class of AMPA receptor allosteric modulators. Part 1: design, synthesis, and SAR of 3-aryl-4-cyano-5-substituted-heteroaryl-2-carboxylic acid derivatives.
Fernandez MC, Castaño A, Dominguez E, Escribano A, Jiang D, Jimenez A, Hong E, Hornback WJ, Nisenbaum ES, Rankl N, Tromiczak E, Vaught G, Zarrinmayeh H, Zimmerman DM.
Bioorg Med Chem Lett (2006) 16 : 5057 -5061
PubMed 16879964 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 20 6.64 7.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL214833 1 7.33
CHEMBL214834 1 7.11
CHEMBL213197 1 7.01
CHEMBL385220 1 6.76
CHEMBL218347 1 6.75
CHEMBL217111 1 6.48
CHEMBL435582 1 6.25
CHEMBL384010 1 6.17
CHEMBL387304 1 5.94
CHEMBL378065 1 -
CHEMBL212021 1 -
CHEMBL213379 1 -
CHEMBL538396 1 -
CHEMBL215987 1 -
CHEMBL385502 1 -
CHEMBL216478 1 -
CHEMBL377662 1 -
CHEMBL334920 1 -
CHEMBL61593 CYCLOTHIAZIDE 4.0 1 -
CHEMBL384175 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL214833 EC50 = 47.0 nM 7.33
CHEMBL214834 EC50 = 78.0 nM 7.11
CHEMBL213197 EC50 = 97.0 nM 7.01
CHEMBL385220 EC50 = 174.0 nM 6.76
CHEMBL218347 EC50 = 176.0 nM 6.75
CHEMBL217111 EC50 = 333.0 nM 6.48
CHEMBL435582 EC50 = 559.0 nM 6.25
CHEMBL384010 EC50 = 684.0 nM 6.17
CHEMBL387304 EC50 = 1137.0 nM 5.94
CHEMBL378065 EC50 - -
CHEMBL212021 EC50 - -
CHEMBL213379 EC50 - -
CHEMBL538396 EC50 - -
CHEMBL215987 EC50 - -
CHEMBL385502 EC50 > 3000.0 nM -
CHEMBL216478 EC50 - -
CHEMBL377662 EC50 - -
CHEMBL334920 EC50 - -
CHEMBL61593 CYCLOTHIAZIDE EC50 - -
CHEMBL384175 EC50 > 3000.0 nM -