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Assay Detail

CHEMBL912250

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant ROCK1 expressed in Sf9 cells at 10 mM
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Expert

Target

Rho-associated protein kinase 1 (CHEMBL3231)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of dihydropyridone indazole amides as selective Rho-kinase inhibitors.
Goodman KB, Cui H, Dowdell SE, Gaitanopoulos DE, Ivy RL, Sehon CA, Stavenger RA, Wang GZ, Viet AQ, Xu W, Ye G, Semus SF, Evans C, Fries HE, Jolivette LJ, Kirkpatrick RB, Dul E, Khandekar SS, Yi T, Jung DK, Wright LL, Smith GK, Behm DJ, Bentley R, Doe CP, Hu E, Lee D.
J Med Chem (2007) 50 : 6 -9
PubMed 17201405 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.69 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL218719 1 8.30
CHEMBL427058 GSK-466317A 1 8.10
CHEMBL374453 GSK-299115A 1 8.10
CHEMBL218720 GSK-466314A 1 8.05
CHEMBL441702 GSK-270822A 1 8.05
CHEMBL384073 GSK-180736A 1 7.85
CHEMBL375312 1 7.85
CHEMBL218654 1 7.29
CHEMBL217910 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL218719 IC50 = 5.0 nM 8.30
CHEMBL427058 GSK-466317A IC50 = 8.0 nM 8.10
CHEMBL374453 GSK-299115A IC50 = 8.0 nM 8.10
CHEMBL218720 GSK-466314A IC50 = 9.0 nM 8.05
CHEMBL441702 GSK-270822A IC50 = 9.0 nM 8.05
CHEMBL384073 GSK-180736A IC50 = 14.0 nM 7.85
CHEMBL375312 IC50 = 14.0 nM 7.85
CHEMBL218654 IC50 = 51.0 nM 7.29
CHEMBL217910 IC50 = 2500.0 nM 5.60