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Assay Detail

CHEMBL925796

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]naloxone from human mu poioid receptor expressed in HEK293 cells
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL233)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Live cell monitoring of mu-opioid receptor-mediated G-protein activation reveals strong biological activity of close morphine biosynthetic precursors.
Nikolaev VO, Boettcher C, Dees C, Bünemann M, Lohse MJ, Zenk MH.
J Biol Chem (2007) 282 : 27126 -27132
PubMed 17616524 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 6.13 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL70 MORPHINE 4.0 1 8.40
CHEMBL255467 MORPHINONE 1 6.84
CHEMBL437602 ORIPAVINE 1 6.54
CHEMBL257627 CODEINONE 1 6.34
CHEMBL485 CODEINE 4.0 1 5.20
CHEMBL403893 THEBAINE 1 5.13
CHEMBL404097 SALUTARIDINE 1 4.46
CHEMBL401501 (R)-RETICULINE 1 -
CHEMBL255882 SALUTARIDINOL 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL70 MORPHINE Ki = 4.0 nM 8.40
CHEMBL255467 MORPHINONE Ki = 146.0 nM 6.84
CHEMBL437602 ORIPAVINE Ki = 286.0 nM 6.54
CHEMBL257627 CODEINONE Ki = 459.0 nM 6.34
CHEMBL485 CODEINE Ki = 6300.0 nM 5.20
CHEMBL403893 THEBAINE Ki = 7400.0 nM 5.13
CHEMBL404097 SALUTARIDINE Ki = 34600.0 nM 4.46
CHEMBL401501 (R)-RETICULINE Ki - -
CHEMBL255882 SALUTARIDINOL Ki - -