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Assay Detail

CHEMBL952617

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay
5
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Jurkat
Confidence 1 — Organism
Curated By Autocuration

Target

Jurkat (CHEMBL397)
Type CELL-LINE
Organism Homo sapiens

Publication

Essential requirement for sphingosine kinase 2 in a sphingolipid apoptosis pathway activated by FTY720 analogues.
Don AS, Martinez-Lamenca C, Webb WR, Proia RL, Roberts E, Rosen H.
J Biol Chem (2007) 282 : 15833 -15842
PubMed 17400555 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.34 5.54
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL67166 SPHINGOSINE 1 5.54
CHEMBL448741 DIHYDROSPINGOSINE 1 5.43
CHEMBL236036 PHYTOSPHINGOSINE 1 5.28
CHEMBL268745 1 5.11
CHEMBL44657 ETOPOSIDE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL67166 SPHINGOSINE IC50 = 2880.0 nM 5.54
CHEMBL448741 DIHYDROSPINGOSINE IC50 = 3680.0 nM 5.43
CHEMBL236036 PHYTOSPHINGOSINE IC50 = 5290.0 nM 5.28
CHEMBL268745 IC50 = 7720.0 nM 5.11
CHEMBL44657 ETOPOSIDE Activity - -