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Assay Detail

CHEMBL1282292

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]chloramphenicol from 70S ribosomal complex in Staphylococcus aureus ATCC 29213 by competitive binding assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Staphylococcus aureus
Subcellular Ribosome
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Bacterial 70S ribosome (CHEMBL2363135)
Type PROTEIN NUCLEIC-ACID COMPLEX
Organism Bacteria

Publication

R chi-01, a new family of oxazolidinones that overcome ribosome-based linezolid resistance.
Skripkin E, McConnell TS, DeVito J, Lawrence L, Ippolito JA, Duffy EM, Sutcliffe J, Franceschi F.
Antimicrob Agents Chemother (2008) 52 : 3550 -3557
PubMed 18663023 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.79 6.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL130 CHLORAMPHENICOL 4.0 1 6.37
CHEMBL1241590 FLORFENICOL 2.0 1 4.70
CHEMBL461534 1 4.35
CHEMBL1276662 1 4.30
CHEMBL1275617 1 4.24
CHEMBL126 LINEZOLID 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL130 CHLORAMPHENICOL IC50 = 430.0 nM 6.37
CHEMBL1241590 FLORFENICOL IC50 = 20000.0 nM 4.70
CHEMBL461534 IC50 = 45000.0 nM 4.35
CHEMBL1276662 IC50 = 50000.0 nM 4.30
CHEMBL1275617 IC50 = 58000.0 nM 4.24
CHEMBL126 LINEZOLID IC50 = 1380000.0 nM -