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Assay Detail

CHEMBL2344595

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PDGFRbeta in human SF539 cells assessed as inhibition of PDGFR-BB-induced tyrosine phosphorylation incubated for 60 mins prior to EGF-activation measured 10 mins by ELISA
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SF-539
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Platelet-derived growth factor receptor beta (CHEMBL1913)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological activity of 5-chloro-N⁴-substituted phenyl-9H-pyrimido[4,5-b]indole-2,4-diamines as vascular endothelial growth factor receptor-2 inhibitors and antiangiogenic agents.
Gangjee A, Zaware N, Raghavan S, Disch BC, Thorpe JE, Bastian A, Ihnat MA.
Bioorg Med Chem (2013) 21 : 1857 -1864
PubMed 23434139 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 4.57 5.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL328710 1 5.43
CHEMBL553 ERLOTINIB 4.0 1 4.91
CHEMBL2337366 1 4.30
CHEMBL2337367 1 4.15
CHEMBL535 SUNITINIB 4.0 1 4.08
CHEMBL2337372 1 -
CHEMBL2337371 1 -
CHEMBL2337370 1 -
CHEMBL2337369 1 -
CHEMBL2337368 1 -
CHEMBL2337365 1 -
CHEMBL2337364 1 -
CHEMBL2337363 1 -
CHEMBL2337362 1 -
CHEMBL2337374 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL328710 IC50 = 3700.0 nM 5.43
CHEMBL553 ERLOTINIB IC50 = 12200.0 nM 4.91
CHEMBL2337366 IC50 = 50500.0 nM 4.30
CHEMBL2337367 IC50 = 70600.0 nM 4.15
CHEMBL535 SUNITINIB IC50 = 83100.0 nM 4.08
CHEMBL2337372 IC50 = 196200.0 nM -
CHEMBL2337371 IC50 > 200000.0 nM -
CHEMBL2337370 IC50 = 170800.0 nM -
CHEMBL2337369 IC50 > 200000.0 nM -
CHEMBL2337368 IC50 > 200000.0 nM -
CHEMBL2337365 IC50 = 172200.0 nM -
CHEMBL2337364 IC50 > 200000.0 nM -
CHEMBL2337363 IC50 = 196200.0 nM -
CHEMBL2337362 IC50 > 200000.0 nM -
CHEMBL2337374 IC50 > 200000.0 nM -