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Assay Detail

CHEMBL3607737

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to 5'-FAM-pre-miR-372 (unknown origin) after 4 hrs by fluorescence assay in presence of 100-fold excess of 15-mer DNA
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Not specified
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Ribosome-targeting antibiotics as inhibitors of oncogenic microRNAs biogenesis: Old scaffolds for new perspectives in RNA targeting.
Tran TP, Vo DD, Di Giorgio A, Duca M.
Bioorg Med Chem (2015) 23 : 5334 -5344
PubMed 26264847 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 11 5.09 5.84

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1434 MINOCYCLINE 4.0 1 5.84
CHEMBL1200699 DOXYCYCLINE 4.0 1 5.79
CHEMBL1909452 APRAMYCIN 2.0 1 5.18
CHEMBL529 AZITHROMYCIN 4.0 1 5.13
CHEMBL6067485 GENTAMICIN 4.0 1 5.09
CHEMBL1517 OXYTETRACYCLINE 4.0 1 4.99
CHEMBL184618 FRAMYCETIN 3.0 1 4.95
CHEMBL532 ERYTHROMYCIN 4.0 1 4.51
CHEMBL372795 STREPTOMYCIN 4.0 1 4.32
CHEMBL370143 PAROMOMYCIN 4.0 1 -
CHEMBL427409 NEAMINE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1434 MINOCYCLINE Kd = 1450.0 nM 5.84
CHEMBL1200699 DOXYCYCLINE Kd = 1620.0 nM 5.79
CHEMBL1909452 APRAMYCIN Kd = 6550.0 nM 5.18
CHEMBL529 AZITHROMYCIN Kd = 7430.0 nM 5.13
CHEMBL6067485 GENTAMICIN Kd = 8100.0 nM 5.09
CHEMBL1517 OXYTETRACYCLINE Kd = 10200.0 nM 4.99
CHEMBL184618 FRAMYCETIN Kd = 11300.0 nM 4.95
CHEMBL532 ERYTHROMYCIN Kd = 30700.0 nM 4.51
CHEMBL372795 STREPTOMYCIN Kd = 48200.0 nM 4.32
CHEMBL370143 PAROMOMYCIN Kd = 167000.0 nM -
CHEMBL427409 NEAMINE Kd = 248000.0 nM -