Assay Detail
Binding
CHEMBL3887452
Review assay metadata, readout intent, target linkage, and publication context from the same page.
In Vitro Assay: Tissue kallikrein-1 (37° C) activity was determined in reactions containing 0.05 nM enzyme and 90 μM substrate (H-D-Val-Leu-Arg-AFC) in buffer (0.1M sodium phosphate pH 7.4, 0.2 M NaCl, 0.5% PEG 8000, and 1% DMSO). Assays were performed using 96-well microtiter plates (COSTAR 3600, CORNING, NY, USA) and a thermostatic temperature controlled plate reader (SPECTRAMAX® Gemini, Molecular Devices, Sunnyvale, Calif., USA). Fluorescence was monitored using 400 nm excitation and 505 nm emission wavelengths.
42
Total Activities
42
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Macrocyclic factor VIIa inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 42 | 6.50 | 7.39 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3906646 | — | — | 1 | 7.39 |
| CHEMBL3977297 | — | — | 1 | 7.30 |
| CHEMBL4114547 | — | — | 1 | 7.16 |
| CHEMBL3915633 | — | — | 1 | 7.16 |
| CHEMBL3917941 | — | — | 1 | 7.02 |
| CHEMBL3930738 | — | — | 1 | 7.00 |
| CHEMBL3909009 | — | — | 1 | 7.00 |
| CHEMBL4108209 | — | — | 1 | 6.96 |
| CHEMBL3908500 | — | — | 1 | 6.92 |
| CHEMBL3922904 | — | — | 1 | 6.75 |
| CHEMBL3969432 | — | — | 1 | 6.72 |
| CHEMBL3973659 | — | — | 1 | 6.70 |
| CHEMBL3903215 | — | — | 1 | 6.68 |
| CHEMBL3902759 | — | — | 1 | 6.64 |
| CHEMBL3891120 | — | — | 1 | 6.57 |
| CHEMBL3939509 | — | — | 1 | 6.57 |
| CHEMBL3901206 | — | — | 1 | 6.57 |
| CHEMBL3920165 | — | — | 1 | 6.54 |
| CHEMBL4106751 | — | — | 1 | 6.54 |
| CHEMBL3986081 | — | — | 1 | 6.54 |
| CHEMBL4113473 | — | — | 1 | 6.52 |
| CHEMBL3892246 | — | — | 1 | 6.50 |
| CHEMBL3979309 | — | — | 1 | 6.50 |
| CHEMBL3897709 | — | — | 1 | 6.48 |
| CHEMBL3956442 | — | — | 1 | 6.42 |
| CHEMBL3980787 | — | — | 1 | 6.41 |
| CHEMBL3983744 | — | — | 1 | 6.40 |
| CHEMBL3913920 | — | — | 1 | 6.31 |
| CHEMBL3970623 | — | — | 1 | 6.26 |
| CHEMBL3895397 | — | — | 1 | 6.26 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3906646 | — | Ki | = | 41.0 | nM | 7.39 |
| CHEMBL3977297 | — | Ki | = | 50.0 | nM | 7.30 |
| CHEMBL3915633 | — | Ki | = | 70.0 | nM | 7.16 |
| CHEMBL4114547 | — | Ki | = | 70.0 | nM | 7.16 |
| CHEMBL3917941 | — | Ki | = | 96.0 | nM | 7.02 |
| CHEMBL3930738 | — | Ki | = | 100.0 | nM | 7.00 |
| CHEMBL3909009 | — | Ki | = | 100.0 | nM | 7.00 |
| CHEMBL4108209 | — | Ki | = | 110.0 | nM | 6.96 |
| CHEMBL3908500 | — | Ki | = | 120.0 | nM | 6.92 |
| CHEMBL3922904 | — | Ki | = | 180.0 | nM | 6.75 |
| CHEMBL3969432 | — | Ki | = | 190.0 | nM | 6.72 |
| CHEMBL3973659 | — | Ki | = | 200.0 | nM | 6.70 |
| CHEMBL3903215 | — | Ki | = | 210.0 | nM | 6.68 |
| CHEMBL3902759 | — | Ki | = | 230.0 | nM | 6.64 |
| CHEMBL3901206 | — | Ki | = | 270.0 | nM | 6.57 |
| CHEMBL3891120 | — | Ki | = | 270.0 | nM | 6.57 |
| CHEMBL3939509 | — | Ki | = | 270.0 | nM | 6.57 |
| CHEMBL3920165 | — | Ki | = | 290.0 | nM | 6.54 |
| CHEMBL4106751 | — | Ki | = | 290.0 | nM | 6.54 |
| CHEMBL3986081 | — | Ki | = | 290.0 | nM | 6.54 |
| CHEMBL4113473 | — | Ki | = | 300.0 | nM | 6.52 |
| CHEMBL3892246 | — | Ki | = | 320.0 | nM | 6.50 |
| CHEMBL3979309 | — | Ki | = | 320.0 | nM | 6.50 |
| CHEMBL3897709 | — | Ki | = | 330.0 | nM | 6.48 |
| CHEMBL3956442 | — | Ki | = | 380.0 | nM | 6.42 |
| CHEMBL3980787 | — | Ki | = | 390.0 | nM | 6.41 |
| CHEMBL3983744 | — | Ki | = | 400.0 | nM | 6.40 |
| CHEMBL3913920 | — | Ki | = | 490.0 | nM | 6.31 |
| CHEMBL3970623 | — | Ki | = | 550.0 | nM | 6.26 |
| CHEMBL3895397 | — | Ki | = | 550.0 | nM | 6.26 |
| CHEMBL3917434 | — | Ki | = | 600.0 | nM | 6.22 |
| CHEMBL3951355 | — | Ki | = | 630.0 | nM | 6.20 |
| CHEMBL3930523 | — | Ki | = | 650.0 | nM | 6.19 |
| CHEMBL3902498 | — | Ki | = | 650.0 | nM | 6.19 |
| CHEMBL3907868 | — | Ki | = | 700.0 | nM | 6.16 |
| CHEMBL3961226 | — | Ki | = | 750.0 | nM | 6.12 |
| CHEMBL3949417 | — | Ki | = | 780.0 | nM | 6.11 |
| CHEMBL3901391 | — | Ki | = | 790.0 | nM | 6.10 |
| CHEMBL3898371 | — | Ki | = | 1100.0 | nM | 5.96 |
| CHEMBL4108610 | — | Ki | = | 1620.0 | nM | 5.79 |
| CHEMBL3967595 | — | Ki | = | 2100.0 | nM | 5.68 |
| CHEMBL4110036 | — | Ki | = | 3330.0 | nM | 5.48 |