Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3991727

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry.
243
Total Activities
243
Compounds Tested
1
Activity Types
1
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type K-562, COLO 205, SK-N-BE(2) and MV-4-11
Subcellular Cell lysate
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glycogen synthase kinase-3 beta (CHEMBL262)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The target landscape of clinical kinase drugs.
Klaeger S, Heinzlmeir S and Wilhelm M et al
Science (2017) 358
PubMed 29191878 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 243 6.44 8.15

Assay Parameters

Parameter Type Value Units Text
Direct Binder Direct Binder - yes

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL495727 AT-9283 2.0 1 8.15
CHEMBL3545083 RGB-286638 1.0 1 8.10
CHEMBL1230607 PHA-793887 1.0 1 7.92
CHEMBL3301610 ABEMACICLIB 4.0 1 7.89
CHEMBL445813 AT-7519 2.0 1 7.24
CHEMBL488436 AZD-5438 1.0 1 7.21
CHEMBL3688339 1 7.10
CHEMBL300138 ENZASTAURIN 3.0 1 6.72
CHEMBL296468 BMS-387032 1.0 1 6.52
CHEMBL494089 GSK-690693 1.0 1 6.37
CHEMBL3301612 ENCORAFENIB 4.0 1 6.37
CHEMBL1944698 ZOTIRACICLIB 2.0 1 6.35
CHEMBL3544964 RAVOXERTINIB 2.0 1 6.22
CHEMBL3128043 PF-03758309 1.0 1 6.18
CHEMBL565612 SOTRASTAURIN 2.0 1 6.15
CHEMBL1084546 PF-00562271 1.0 1 6.15
CHEMBL428690 ALVOCIDIB 3.0 1 6.13
CHEMBL571948 Y-39983 1.0 1 6.03
CHEMBL3137331 DEFACTINIB 3.0 1 5.88
CHEMBL2103840 DINACICLIB 3.0 1 5.84
CHEMBL3991935 1 5.57
CHEMBL1230165 SILMITASERTIB 2.0 1 5.31
CHEMBL91829 RUBOXISTAURIN 3.0 1 5.30
CHEMBL3544932 TAK-901 1.0 1 5.21
CHEMBL2030402 1 5.06
CHEMBL2103851 AMUVATINIB 2.0 1 -
CHEMBL3545076 LY-2584702 1.0 1 -
CHEMBL2220486 LUCITANIB 2.0 1 -
CHEMBL1233528 VOLASERTIB 3.0 1 -
CHEMBL482967 CYC-116 1.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL495727 AT-9283 Kd = 7.0 nM 8.15
CHEMBL3545083 RGB-286638 Kd = 8.0 nM 8.10
CHEMBL1230607 PHA-793887 Kd = 12.0 nM 7.92
CHEMBL3301610 ABEMACICLIB Kd = 13.0 nM 7.89
CHEMBL445813 AT-7519 Kd = 58.0 nM 7.24
CHEMBL488436 AZD-5438 Kd = 62.0 nM 7.21
CHEMBL3688339 Kd = 80.0 nM 7.10
CHEMBL300138 ENZASTAURIN Kd = 190.0 nM 6.72
CHEMBL296468 BMS-387032 Kd = 299.0 nM 6.52
CHEMBL3301612 ENCORAFENIB Kd = 425.0 nM 6.37
CHEMBL494089 GSK-690693 Kd = 428.0 nM 6.37
CHEMBL1944698 ZOTIRACICLIB Kd = 447.0 nM 6.35
CHEMBL3544964 RAVOXERTINIB Kd = 599.0 nM 6.22
CHEMBL3128043 PF-03758309 Kd = 667.0 nM 6.18
CHEMBL1084546 PF-00562271 Kd = 707.0 nM 6.15
CHEMBL565612 SOTRASTAURIN Kd = 709.0 nM 6.15
CHEMBL428690 ALVOCIDIB Kd = 740.0 nM 6.13
CHEMBL571948 Y-39983 Kd = 924.0 nM 6.03
CHEMBL3137331 DEFACTINIB Kd = 1322.0 nM 5.88
CHEMBL2103840 DINACICLIB Kd = 1430.0 nM 5.84
CHEMBL3991935 Kd = 2722.0 nM 5.57
CHEMBL1230165 SILMITASERTIB Kd = 4850.0 nM 5.31
CHEMBL91829 RUBOXISTAURIN Kd = 5063.0 nM 5.30
CHEMBL3544932 TAK-901 Kd = 6168.0 nM 5.21
CHEMBL2030402 Kd = 8793.0 nM 5.06
CHEMBL1236962 OMIPALISIB Kd > 30000.0 nM -
CHEMBL1908394 GSK-461364 Kd > 30000.0 nM -
CHEMBL3182621 AZD-8330 Kd > 30000.0 nM -
CHEMBL3545085 XL-228 Kd > 30000.0 nM -
CHEMBL1336 SORAFENIB Kd > 30000.0 nM -
CHEMBL206834 BAFETINIB Kd > 30000.0 nM -
CHEMBL2133806 JNJ-38877605 Kd > 30000.0 nM -
CHEMBL522892 DOVITINIB Kd > 30000.0 nM -
CHEMBL400402 Kd > 30000.0 nM -
CHEMBL939 GEFITINIB Kd > 30000.0 nM -
CHEMBL1088751 PH-797804 Kd > 30000.0 nM -
CHEMBL2219422 AFURESERTIB Kd > 30000.0 nM -
CHEMBL101253 VATALANIB Kd > 30000.0 nM -
CHEMBL2105717 CABOZANTINIB Kd > 30000.0 nM -
CHEMBL1822792 MK-2461 Kd > 30000.0 nM -
CHEMBL2105728 CRENOLANIB Kd > 30000.0 nM -
CHEMBL377300 BRIVANIB Kd > 30000.0 nM -
CHEMBL413 SIROLIMUS Kd > 30000.0 nM -
CHEMBL405130 WHI-P131 Kd > 30000.0 nM -
CHEMBL2103875 TRAMETINIB Kd > 30000.0 nM -
CHEMBL372764 PERIFOSINE Kd > 30000.0 nM -
CHEMBL483158 ALISERTIB Kd > 30000.0 nM -
CHEMBL3545307 MERESTINIB Kd > 30000.0 nM -
CHEMBL607707 PELITINIB Kd > 30000.0 nM -
CHEMBL475251 R-406 Kd > 30000.0 nM -