Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3991905

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry.
243
Total Activities
243
Compounds Tested
1
Activity Types
1
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type K-562, COLO 205, SK-N-BE(2) and MV-4-11
Subcellular Cell lysate
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase TBK1 (CHEMBL5408)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The target landscape of clinical kinase drugs.
Klaeger S, Heinzlmeir S and Wilhelm M et al
Science (2017) 358
PubMed 29191878 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 243 6.01 7.11

Assay Parameters

Parameter Type Value Units Text
Direct Binder Direct Binder - yes

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4289017 PF-03814735 1.0 1 7.11
CHEMBL495727 AT-9283 2.0 1 6.99
CHEMBL608533 MIDOSTAURIN 4.0 1 6.73
CHEMBL3991929 Cerdulatinib Hydrochloride 1 6.52
CHEMBL475251 R-406 2.0 1 6.46
CHEMBL3128043 PF-03758309 1.0 1 6.42
CHEMBL574737 UCN-01 2.0 1 6.41
CHEMBL3688339 1 6.34
CHEMBL3545328 XL-019 1.0 1 6.25
CHEMBL1078178 MOMELOTINIB 4.0 1 6.25
CHEMBL3699142 1 6.12
CHEMBL603469 LESTAURTINIB 3.0 1 6.01
CHEMBL288441 BOSUTINIB 4.0 1 5.99
CHEMBL3545085 XL-228 1.0 1 5.95
CHEMBL522892 DOVITINIB 3.0 1 5.95
CHEMBL281948 K-252A 1 5.94
CHEMBL482967 CYC-116 1.0 1 5.93
CHEMBL1721885 SU-014813 2.0 1 5.88
CHEMBL535 SUNITINIB 4.0 1 5.82
CHEMBL1976040 ADAVOSERTIB 2.0 1 5.79
CHEMBL428690 ALVOCIDIB 3.0 1 5.71
CHEMBL1908397 KW-2449 1.0 1 5.71
CHEMBL274654 ORANTINIB 3.0 1 5.70
CHEMBL3545083 RGB-286638 1.0 1 5.70
CHEMBL564829 MILCICLIB 2.0 1 5.68
CHEMBL1287853 FEDRATINIB 4.0 1 5.65
CHEMBL3544932 TAK-901 1.0 1 5.56
CHEMBL502835 NINTEDANIB 4.0 1 5.28
CHEMBL2035187 PACRITINIB 4.0 1 4.30
CHEMBL2087361 ICOTINIB 3.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4289017 PF-03814735 Kd = 78.0 nM 7.11
CHEMBL495727 AT-9283 Kd = 102.0 nM 6.99
CHEMBL608533 MIDOSTAURIN Kd = 188.0 nM 6.73
CHEMBL3991929 Cerdulatinib Hydrochloride Kd = 299.0 nM 6.52
CHEMBL475251 R-406 Kd = 345.0 nM 6.46
CHEMBL3128043 PF-03758309 Kd = 377.0 nM 6.42
CHEMBL574737 UCN-01 Kd = 393.0 nM 6.41
CHEMBL3688339 Kd = 452.0 nM 6.34
CHEMBL1078178 MOMELOTINIB Kd = 562.0 nM 6.25
CHEMBL3545328 XL-019 Kd = 561.0 nM 6.25
CHEMBL3699142 Kd = 759.0 nM 6.12
CHEMBL603469 LESTAURTINIB Kd = 977.0 nM 6.01
CHEMBL288441 BOSUTINIB Kd = 1022.0 nM 5.99
CHEMBL3545085 XL-228 Kd = 1118.0 nM 5.95
CHEMBL522892 DOVITINIB Kd = 1114.0 nM 5.95
CHEMBL281948 K-252A Kd = 1157.0 nM 5.94
CHEMBL482967 CYC-116 Kd = 1162.0 nM 5.93
CHEMBL1721885 SU-014813 Kd = 1330.0 nM 5.88
CHEMBL535 SUNITINIB Kd = 1502.0 nM 5.82
CHEMBL1976040 ADAVOSERTIB Kd = 1629.0 nM 5.79
CHEMBL428690 ALVOCIDIB Kd = 1949.0 nM 5.71
CHEMBL1908397 KW-2449 Kd = 1966.0 nM 5.71
CHEMBL274654 ORANTINIB Kd = 1982.0 nM 5.70
CHEMBL3545083 RGB-286638 Kd = 2007.0 nM 5.70
CHEMBL564829 MILCICLIB Kd = 2088.0 nM 5.68
CHEMBL1287853 FEDRATINIB Kd = 2230.0 nM 5.65
CHEMBL3544932 TAK-901 Kd = 2762.0 nM 5.56
CHEMBL502835 NINTEDANIB Kd = 5306.0 nM 5.28
CHEMBL2035187 PACRITINIB Kd = 49601.0 nM 4.30
CHEMBL3184679 AZD-4547 Kd > 30000.0 nM -
CHEMBL1236107 SGX-523 Kd > 30000.0 nM -
CHEMBL521851 PICTILISIB Kd > 30000.0 nM -
CHEMBL2029988 CEP-32496 Kd > 30000.0 nM -
CHEMBL1230165 SILMITASERTIB Kd > 30000.0 nM -
CHEMBL14762 SELICICLIB Kd > 30000.0 nM -
CHEMBL2133806 JNJ-38877605 Kd > 30000.0 nM -
CHEMBL2105717 CABOZANTINIB Kd > 30000.0 nM -
CHEMBL558752 RAF-265 Kd > 30000.0 nM -
CHEMBL3264002 AVUTOMETINIB Kd > 30000.0 nM -
CHEMBL3545157 TIDEGLUSIB Kd > 30000.0 nM -
CHEMBL488436 AZD-5438 Kd > 30000.0 nM -
CHEMBL3186534 RIVOCERANIB Kd > 30000.0 nM -
CHEMBL2105759 BARICITINIB Kd > 30000.0 nM -
CHEMBL2220486 LUCITANIB Kd > 30000.0 nM -
CHEMBL1088752 LOSMAPIMOD Kd > 30000.0 nM -
CHEMBL1231124 AZD-1480 Kd > 30000.0 nM -
CHEMBL215152 DEFOSBARASERTIB Kd > 30000.0 nM -
CHEMBL1233528 VOLASERTIB Kd > 30000.0 nM -
CHEMBL445813 AT-7519 Kd > 30000.0 nM -
CHEMBL3545308 ROCILETINIB Kd > 30000.0 nM -