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Assay Detail

CHEMBL4422501

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of poly-histidine and GST-tagged full length human Eya3 isoform 2 (127 to 573 residues) using para-nitrophenol phosphate substrate incubated for 30 mins
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Eyes absent homolog 3 (CHEMBL4296245)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Use of small molecule inhibitors targeting EYA tyrosine phosphatase
(2017)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 4.77 5.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3526986 1 5.51
CHEMBL232201 BENZIODARONE 4.0 1 5.47
CHEMBL389052 1 5.08
CHEMBL388590 BENZBROMARONE 4.0 1 5.08
CHEMBL4290672 1 4.86
CHEMBL1523279 1 4.82
CHEMBL4281427 1 4.79
CHEMBL1474963 BENZARONE 4.0 1 4.77
CHEMBL4287926 1 4.76
CHEMBL1767079 1 4.57
CHEMBL1767107 1 4.39
CHEMBL224006 POZTINURAD 2.0 1 4.38
CHEMBL1767090 1 4.14
CHEMBL374618 1 4.09

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3526986 IC50 = 3100.0 nM 5.51
CHEMBL232201 BENZIODARONE IC50 = 3400.0 nM 5.47
CHEMBL389052 IC50 = 8300.0 nM 5.08
CHEMBL388590 BENZBROMARONE IC50 = 8300.0 nM 5.08
CHEMBL4290672 IC50 = 13800.0 nM 4.86
CHEMBL1523279 IC50 = 15200.0 nM 4.82
CHEMBL4281427 IC50 = 16400.0 nM 4.79
CHEMBL1474963 BENZARONE IC50 = 17000.0 nM 4.77
CHEMBL4287926 IC50 = 17500.0 nM 4.76
CHEMBL1767079 IC50 = 27200.0 nM 4.57
CHEMBL1767107 IC50 = 40800.0 nM 4.39
CHEMBL224006 POZTINURAD IC50 = 41900.0 nM 4.38
CHEMBL1767090 IC50 = 73100.0 nM 4.14
CHEMBL374618 IC50 = 81100.0 nM 4.09