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Assay Detail

CHEMBL4880035

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Binding
GPCRScan assay: inhibition of D2
203
Total Activities
181
Compounds Tested
2
Activity Types
12
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(2) dopamine receptor (CHEMBL217)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

SGC PDSP GPCRScan assays
SGC Frankfurt
(2021)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Inhibition 181 - -
Ki 22 5.83 6.48

Assay Parameters

Parameter Type Value Units Text
Parental cells Parental cells - stable fibroblast
PMID PMID - 1977888/26685100
Protein length Protein length - full-length
Radioligand conc. used [nM] Radioligand conc. used [nM] - 0.4 – 1.0
Radioligand Kd Radioligand Kd 0.47 nM
Radioligand name Radioligand name - [3H]-N-methylspiperone
Radioligand pKd Radioligand pKd 9.33
Radioligand pKd ± SEM Radioligand pKd ± SEM 0.06
Reference ligand Reference ligand - Haloperidol
Reference ligand Ki Reference ligand Ki 7.15 nM
Reference ligand pKi Reference ligand pKi 8.15
Reference ligand pKi ± SEM Reference ligand pKi ± SEM 0.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4522930 BI-605906 3 6.48
CHEMBL4520293 (R)-ZINC-3573 3 6.45
CHEMBL4456585 WM-1119 3 6.41
CHEMBL4637208 GSK973 3 6.09
CHEMBL4558518 GSI-NC 3 5.91
CHEMBL4520788 BAY-294 3 5.70
CHEMBL4576555 T3-CLK-N 3 5.63
CHEMBL561132 MK-7246 1.0 3 5.43
CHEMBL3480577 UCSF924 3 5.40
CHEMBL4526351 BAY-309 3 5.00
CHEMBL4522773 BAY-827 1 -
CHEMBL4585243 BAY-7598 1 -
CHEMBL4539943 BAY-694 1 -
CHEMBL4521594 BAY-1797 1 -
CHEMBL4516843 BAY-207 1 -
CHEMBL4537788 BAY-784 1 -
CHEMBL4564312 BAY-786 1 -
CHEMBL4448899 BAY-876 1 -
CHEMBL4552180 BAY-588 1 -
CHEMBL4445670 BAY-885 1 -
CHEMBL4593677 BAY-474 1 -
CHEMBL4066040 A-1596584 1 -
CHEMBL4093612 A-1596586 1 -
CHEMBL332794 A-192621 1 -
CHEMBL4513786 A-545 1 -
CHEMBL111612 ABT-546 1 -
CHEMBL4526341 A-108 1 -
CHEMBL4586794 ABT-100 1 -
CHEMBL3701197 A-226 1 -
CHEMBL3697701 A-079 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4522930 BI-605906 Ki = 331.13 nM 6.48
CHEMBL4522930 BI-605906 Ki = 341.44 nM 6.47
CHEMBL4520293 (R)-ZINC-3573 Ki = 354.81 nM 6.45
CHEMBL4520293 (R)-ZINC-3573 Ki = 369.37 nM 6.43
CHEMBL4456585 WM-1119 Ki = 389.05 nM 6.41
CHEMBL4456585 WM-1119 Ki = 397.52 nM 6.40
CHEMBL4637208 GSK973 Ki = 812.83 nM 6.09
CHEMBL4637208 GSK973 Ki = 967.0 nM 6.01
CHEMBL4558518 GSI-NC Ki = 1219.0 nM 5.91
CHEMBL4558518 GSI-NC Ki = 1258.93 nM 5.90
CHEMBL4520788 BAY-294 Ki = 1995.26 nM 5.70
CHEMBL4576555 T3-CLK-N Ki = 2344.23 nM 5.63
CHEMBL4520788 BAY-294 Ki = 2333.64 nM 5.63
CHEMBL4576555 T3-CLK-N Ki = 2752.36 nM 5.56
CHEMBL561132 MK-7246 Ki = 3746.0 nM 5.43
CHEMBL3480577 UCSF924 Ki = 3981.07 nM 5.40
CHEMBL561132 MK-7246 Ki = 3981.07 nM 5.40
CHEMBL3480577 UCSF924 Ki = 4328.0 nM 5.36
CHEMBL4526351 BAY-309 Ki = 10000.0 nM 5.00
CHEMBL4526351 BAY-309 Ki = 10000.0 nM 5.00
CHEMBL4538591 BAY-440 Inhibition = 10.62 % -
CHEMBL4580220 BAY-3827 Inhibition = -10.36 % -
CHEMBL4630642 BAY-974 Inhibition = -13.67 % -
CHEMBL4456585 WM-1119 Inhibition = 79.62 % -
CHEMBL4520293 (R)-ZINC-3573 Inhibition = 69.78 % -
CHEMBL4534980 (S)-ZINC-3573 Inhibition = 2.85 % -
CHEMBL4460070 ZINC32547799 Inhibition = -7.4 % -
CHEMBL4088216 TP-030-1 Inhibition = -5.46 % -
CHEMBL4549667 TP-030-2 Inhibition = -5.95 % -
CHEMBL4551635 BAY-069 Inhibition = -12.15 % -
CHEMBL4643852 BAY-6672 Inhibition = 9.71 % -
CHEMBL3623630 Inhibition = 50.0 % -
CHEMBL4647642 BAY-173 Inhibition = -16.5 % -
CHEMBL4075278 Inhibition = 2.86 % -
CHEMBL4638406 BAY-3153 Inhibition = 7.26 % -
CHEMBL4514203 BAY-390 Inhibition = -22.13 % -
CHEMBL4536197 A-486 Inhibition = -8.75 % -
CHEMBL4798829 Inhibition = 5.5 % -
CHEMBL4545807 BAY-070 Inhibition = 44.65 % -
CHEMBL4639128 GSK778 Inhibition = 27.74 % -
CHEMBL4637208 GSK973 Inhibition = 60.9 % -
CHEMBL4638939 GSK943 Inhibition = -10.18 % -
CHEMBL1800285 MSD-M1PAM Inhibition = -16.84 % -
CHEMBL2204283 MSD-M1PAM-NC Inhibition = -5.66 % -
CHEMBL3286797 PF-04418948 Inhibition = -23.45 % -
CHEMBL4552554 PF-04475866 Inhibition = -29.44 % -
CHEMBL4530740 BI 1950 Inhibition = 7.62 % -
CHEMBL4577237 BI 9446 Inhibition = 16.44 % -
CHEMBL3137331 DEFACTINIB Inhibition = -33.27 % -
CHEMBL4063249 TP-021n Inhibition = -18.93 % -