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Assay Detail

CHEMBL4880046

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Binding
GPCRScan assay: inhibition of H1
199
Total Activities
179
Compounds Tested
2
Activity Types
12
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H1 receptor (CHEMBL231)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

SGC PDSP GPCRScan assays
SGC Frankfurt
(2021)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Inhibition 179 - -
Ki 20 5.82 7.73

Assay Parameters

Parameter Type Value Units Text
Parental cells Parental cells - stable HEK
PMID PMID - 9794809/7925364
Protein length Protein length - full-length
Radioligand conc. used [nM] Radioligand conc. used [nM] - 0.6 – 2.0
Radioligand Kd Radioligand Kd 0.97 nM
Radioligand name Radioligand name - [3H]-Pyrilamine
Radioligand pKd Radioligand pKd 9.01
Radioligand pKd ± SEM Radioligand pKd ± SEM 0.05
Reference ligand Reference ligand - Chlorpheniramine
Reference ligand Ki Reference ligand Ki 1.64 nM
Reference ligand pKi Reference ligand pKi 8.78
Reference ligand pKi ± SEM Reference ligand pKi ± SEM 0.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3337895 T-26f 3 7.73
CHEMBL3337894 T-26c 3 7.01
CHEMBL4576555 T3-CLK-N 3 5.88
CHEMBL4520788 BAY-294 3 5.87
CHEMBL4531334 T3-CLK 3 5.67
CHEMBL3480577 UCSF924 3 5.34
CHEMBL4586794 ABT-100 3 5.00
CHEMBL111612 ABT-546 3 5.00
CHEMBL4448899 BAY-876 3 5.00
CHEMBL4516843 BAY-207 1 -
CHEMBL4537788 BAY-784 1 -
CHEMBL4564312 BAY-786 1 -
CHEMBL4552180 BAY-588 1 -
CHEMBL4445670 BAY-885 1 -
CHEMBL4455582 BAY-693 1 -
CHEMBL4630644 BAY-386 1 -
CHEMBL4630643 BAY-448 1 -
CHEMBL3907057 BAY-826 1 -
CHEMBL4096813 1 -
CHEMBL4521594 BAY-1797 1 -
CHEMBL4539943 BAY-694 1 -
CHEMBL4585243 BAY-7598 1 -
CHEMBL4593677 BAY-474 1 -
CHEMBL4066040 A-1596584 1 -
CHEMBL4093612 A-1596586 1 -
CHEMBL332794 A-192621 1 -
CHEMBL4513786 A-545 1 -
CHEMBL4526341 A-108 1 -
CHEMBL3701197 A-226 1 -
CHEMBL3697701 A-079 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3337895 T-26f Ki = 18.62 nM 7.73
CHEMBL3337895 T-26f Ki = 19.0 nM 7.72
CHEMBL3337894 T-26c Ki = 98.0 nM 7.01
CHEMBL3337894 T-26c Ki = 100.0 nM 7.00
CHEMBL4576555 T3-CLK-N Ki = 1318.26 nM 5.88
CHEMBL4520788 BAY-294 Ki = 1365.75 nM 5.87
CHEMBL4520788 BAY-294 Ki = 1348.96 nM 5.87
CHEMBL4576555 T3-CLK-N Ki = 1901.2 nM 5.72
CHEMBL4531334 T3-CLK Ki = 2137.96 nM 5.67
CHEMBL4531334 T3-CLK Ki = 2154.79 nM 5.67
CHEMBL3480577 UCSF924 Ki = 4553.0 nM 5.34
CHEMBL3480577 UCSF924 Ki = 4677.35 nM 5.33
CHEMBL4448899 BAY-876 Ki = 10000.0 nM 5.00
CHEMBL4448899 BAY-876 Ki = 10000.0 nM 5.00
CHEMBL111612 ABT-546 Ki = 10000.0 nM 5.00
CHEMBL4586794 ABT-100 Ki = 10000.0 nM 5.00
CHEMBL111612 ABT-546 Ki = 10000.0 nM 5.00
CHEMBL4586794 ABT-100 Ki = 10000.0 nM 5.00
CHEMBL4516611 BTZO-1 Inhibition = -14.85 % -
CHEMBL4063249 TP-021n Inhibition = 14.73 % -
CHEMBL4075278 Inhibition = 16.99 % -
CHEMBL4647642 BAY-173 Inhibition = 14.28 % -
CHEMBL4088216 TP-030-1 Inhibition = 20.53 % -
CHEMBL4458424 BAY-899 Inhibition = 5.89 % -
CHEMBL4592872 BAY-897 Inhibition = 15.89 % -
CHEMBL4537673 BAY-985 Inhibition = 35.0 % -
CHEMBL4538591 BAY-440 Inhibition = 26.36 % -
CHEMBL4580220 BAY-3827 Inhibition = 6.23 % -
CHEMBL4630642 BAY-974 Inhibition = 0.94 % -
CHEMBL4456585 WM-1119 Inhibition = -8.33 % -
CHEMBL4520293 (R)-ZINC-3573 Inhibition = 39.34 % -
CHEMBL4534980 (S)-ZINC-3573 Inhibition = 16.43 % -
CHEMBL4460070 ZINC32547799 Inhibition = -5.64 % -
CHEMBL4636881 GSK046 Inhibition = -10.65 % -
CHEMBL4561806 BTZO-4 Inhibition = 49.86 % -
CHEMBL4282264 A-485 Inhibition = 1.99 % -
CHEMBL4536197 A-486 Inhibition = -7.68 % -
CHEMBL4798829 Inhibition = 37.38 % -
CHEMBL4545807 BAY-070 Inhibition = 0.44 % -
CHEMBL4639128 GSK778 Inhibition = 0.33 % -
CHEMBL4637208 GSK973 Inhibition = -26.88 % -
CHEMBL4638939 GSK943 Inhibition = -1.58 % -
CHEMBL1800285 MSD-M1PAM Inhibition = 28.43 % -
CHEMBL2204283 MSD-M1PAM-NC Inhibition = 9.7 % -
CHEMBL3286797 PF-04418948 Inhibition = -11.53 % -
CHEMBL4552554 PF-04475866 Inhibition = -4.38 % -
CHEMBL4530740 BI 1950 Inhibition = 3.2 % -
CHEMBL4577237 BI 9446 Inhibition = 10.26 % -
CHEMBL4544690 BI 2049 Inhibition = 3.07 % -
CHEMBL3137331 DEFACTINIB Inhibition = -3.38 % -