Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5100323

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal His-Avi tagged recombinant human FGFR3 (447 to 761 residues) expressed in an Sf9 infected baculovirus expression system using biotin-EQEDEPEGDYFEWLE-amide as substrate preincubated for 5 to 10 mins followed by substrate addition and measured after 1 hr by HTRF assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fibroblast growth factor receptor 3 (CHEMBL2742)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Potent and Selective Inhibitors of Wild-Type and Gatekeeper Mutant Fibroblast Growth Factor Receptor (FGFR) 2/3.
Shvartsbart A, Roach JJ, Witten MR, Koblish H, Harris JJ, Covington M, Hess R, Lin L, Frascella M, Truong L, Leffet L, Conlen P, Beshad E, Klabe R, Katiyar K, Kaldon L, Young-Sciame R, He X, Petusky S, Chen KJ, Horsey A, Lei HT, Epling LB, Deller MC, Vechorkin O, Yao W.
J Med Chem (2022) 65 : 15433 -15442
PubMed 36356320 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 8.17 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5175256 1 9.70
CHEMBL5203027 1 9.40
CHEMBL5180631 1 9.30
CHEMBL5189383 1 9.22
CHEMBL5174279 1 9.22
CHEMBL5206837 1 9.15
CHEMBL5184953 1 9.05
CHEMBL5200643 1 9.05
CHEMBL5199231 1 9.00
CHEMBL5202943 1 8.96
CHEMBL5187371 1 8.89
CHEMBL4297522 PEMIGATINIB 4.0 1 8.77
CHEMBL5208443 1 8.66
CHEMBL5196280 1 8.62
CHEMBL5172121 1 8.59
CHEMBL5202408 1 8.59
CHEMBL1852688 INFIGRATINIB 4.0 1 8.57
CHEMBL5201556 1 8.34
CHEMBL5176738 1 7.89
CHEMBL5169540 1 7.75
CHEMBL5170079 1 7.47
CHEMBL5179197 1 7.39
CHEMBL5170742 1 7.27
CHEMBL5180065 1 7.25
CHEMBL5189074 1 7.17
CHEMBL5184462 1 6.78
CHEMBL5190948 1 6.61
CHEMBL5181232 1 6.36
CHEMBL5204157 1 6.29
CHEMBL5170159 1 5.93

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5175256 IC50 = 0.2 nM 9.70
CHEMBL5203027 IC50 = 0.4 nM 9.40
CHEMBL5180631 IC50 = 0.5 nM 9.30
CHEMBL5189383 IC50 = 0.6 nM 9.22
CHEMBL5174279 IC50 = 0.6 nM 9.22
CHEMBL5206837 IC50 = 0.7 nM 9.15
CHEMBL5184953 IC50 = 0.9 nM 9.05
CHEMBL5200643 IC50 = 0.9 nM 9.05
CHEMBL5199231 IC50 = 1.0 nM 9.00
CHEMBL5202943 IC50 = 1.1 nM 8.96
CHEMBL5187371 IC50 = 1.3 nM 8.89
CHEMBL4297522 PEMIGATINIB IC50 = 1.7 nM 8.77
CHEMBL5208443 IC50 = 2.2 nM 8.66
CHEMBL5196280 IC50 = 2.4 nM 8.62
CHEMBL5172121 IC50 = 2.6 nM 8.59
CHEMBL5202408 IC50 = 2.6 nM 8.59
CHEMBL1852688 INFIGRATINIB IC50 = 2.7 nM 8.57
CHEMBL5201556 IC50 = 4.6 nM 8.34
CHEMBL5176738 IC50 = 13.0 nM 7.89
CHEMBL5169540 IC50 = 18.0 nM 7.75
CHEMBL5170079 IC50 = 34.0 nM 7.47
CHEMBL5179197 IC50 = 41.0 nM 7.39
CHEMBL5170742 IC50 = 54.0 nM 7.27
CHEMBL5180065 IC50 = 56.0 nM 7.25
CHEMBL5189074 IC50 = 68.0 nM 7.17
CHEMBL5184462 IC50 = 167.0 nM 6.78
CHEMBL5190948 IC50 = 247.0 nM 6.61
CHEMBL5181232 IC50 = 441.0 nM 6.36
CHEMBL5204157 IC50 = 515.0 nM 6.29
CHEMBL5170159 IC50 = 1177.0 nM 5.93