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Assay Detail

CHEMBL5256647

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant cytoplasmic domain FGFR2 (8 to 134 residues) incubated for 120 mins by mobility shift assay
18
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fibroblast growth factor receptor 2 (CHEMBL4142)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Futibatinib: The First Covalent FGFR Kinase Inhibitor in Clinical Use.
Ito S, Otsuki S, Ohsawa H, Hirano A, Kazuno H, Yamashita S, Egami K, Shibata Y, Yamamiya I, Yamashita F, Kodama Y, Funabashi K, Kazuno H, Komori T, Suzuki S, Sootome H, Hirai H, Sagara T.
ACS Med Chem Lett (2023) 14 : 396 -404
PubMed 37077386 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.34 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3701238 FUTIBATINIB 4.0 2 9.00
CHEMBL3701241 1 8.82
CHEMBL3701243 1 8.47
CHEMBL3701255 1 7.96
CHEMBL5289003 1 7.77
CHEMBL5278505 1 7.66
CHEMBL5287545 1 7.54
CHEMBL5269016 1 7.47
CHEMBL5265881 1 7.24
CHEMBL5268467 1 6.68
CHEMBL5284165 1 6.68
CHEMBL5273555 1 6.62
CHEMBL3701282 1 6.61
CHEMBL5272088 1 6.59
CHEMBL5291403 1 6.50
CHEMBL5275918 1 6.17
CHEMBL5291034 1 5.65

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3701238 FUTIBATINIB IC50 = 1.0 nM 9.00
CHEMBL3701241 IC50 = 1.5 nM 8.82
CHEMBL3701238 FUTIBATINIB IC50 = 1.8 nM 8.74
CHEMBL3701243 IC50 = 3.4 nM 8.47
CHEMBL3701255 IC50 = 11.0 nM 7.96
CHEMBL5289003 IC50 = 17.0 nM 7.77
CHEMBL5278505 IC50 = 22.0 nM 7.66
CHEMBL5287545 IC50 = 29.0 nM 7.54
CHEMBL5269016 IC50 = 34.0 nM 7.47
CHEMBL5265881 IC50 = 57.0 nM 7.24
CHEMBL5284165 IC50 = 207.0 nM 6.68
CHEMBL5268467 IC50 = 207.0 nM 6.68
CHEMBL5273555 IC50 = 239.0 nM 6.62
CHEMBL3701282 IC50 = 244.0 nM 6.61
CHEMBL5272088 IC50 = 255.0 nM 6.59
CHEMBL5291403 IC50 = 313.0 nM 6.50
CHEMBL5275918 IC50 = 670.0 nM 6.17
CHEMBL5291034 IC50 = 2260.0 nM 5.65