Assay Detail
Binding
CHEMBL5442178
Review assay metadata, readout intent, target linkage, and publication context from the same page.
PDSP Secondary Binding target: HTR1A - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | PDSP assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
EUbOPEN Chemogenomics Library - PDSP Secondary Binding
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 22 | 6.47 | 7.63 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2105760 | BREXPIPRAZOLE | 4.0 | 1 | 7.63 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | 4.0 | 1 | 7.50 |
| CHEMBL1256863 | — | — | 1 | 7.41 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | 4.0 | 1 | 7.41 |
| CHEMBL24778 | SILODOSIN | 4.0 | 1 | 7.33 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | 4.0 | 1 | 7.27 |
| CHEMBL540612 | — | — | 1 | 7.25 |
| CHEMBL300555 | SPIROXATRINE | 2.0 | 1 | 7.25 |
| CHEMBL1516474 | TEGASEROD MALEATE | 4.0 | 1 | 7.08 |
| CHEMBL500 | PINDOLOL | 4.0 | 1 | 6.81 |
| CHEMBL79834 | SPIRAMIDE | 2.0 | 1 | 6.41 |
| CHEMBL83894 | FANANSERIN | 2.0 | 1 | 6.29 |
| CHEMBL405355 | NIGULDIPINE | 2.0 | 1 | 6.27 |
| CHEMBL1671 | PROPRANOLOL HYDROCHLORIDE | 4.0 | 1 | 6.19 |
| CHEMBL2107572 | TECALCET HYDROCHLORIDE | 2.0 | 1 | 5.84 |
| CHEMBL2138684 | FROVATRIPTAN SUCCINATE | 4.0 | 1 | 5.83 |
| CHEMBL1200776 | CINACALCET HYDROCHLORIDE | 4.0 | 1 | 5.71 |
| CHEMBL2164327 | — | — | 1 | 5.62 |
| CHEMBL4743495 | — | — | 1 | 5.60 |
| CHEMBL2435402 | — | — | 1 | 5.49 |
| CHEMBL4743049 | — | — | 1 | 5.13 |
| CHEMBL3215877 | — | — | 1 | 5.12 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2105760 | BREXPIPRAZOLE | Ki | = | 23.24 | nM | 7.63 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | Ki | = | 31.64 | nM | 7.50 |
| CHEMBL1256863 | — | Ki | = | 39.2 | nM | 7.41 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | Ki | = | 39.06 | nM | 7.41 |
| CHEMBL24778 | SILODOSIN | Ki | = | 46.97 | nM | 7.33 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | Ki | = | 53.67 | nM | 7.27 |
| CHEMBL540612 | — | Ki | = | 56.16 | nM | 7.25 |
| CHEMBL300555 | SPIROXATRINE | Ki | = | 56.64 | nM | 7.25 |
| CHEMBL1516474 | TEGASEROD MALEATE | Ki | = | 83.91 | nM | 7.08 |
| CHEMBL500 | PINDOLOL | Ki | = | 153.64 | nM | 6.81 |
| CHEMBL79834 | SPIRAMIDE | Ki | = | 388.77 | nM | 6.41 |
| CHEMBL83894 | FANANSERIN | Ki | = | 517.91 | nM | 6.29 |
| CHEMBL405355 | NIGULDIPINE | Ki | = | 538.52 | nM | 6.27 |
| CHEMBL1671 | PROPRANOLOL HYDROCHLORIDE | Ki | = | 644.31 | nM | 6.19 |
| CHEMBL2107572 | TECALCET HYDROCHLORIDE | Ki | = | 1444.44 | nM | 5.84 |
| CHEMBL2138684 | FROVATRIPTAN SUCCINATE | Ki | = | 1471.97 | nM | 5.83 |
| CHEMBL1200776 | CINACALCET HYDROCHLORIDE | Ki | = | 1962.68 | nM | 5.71 |
| CHEMBL2164327 | — | Ki | = | 2423.26 | nM | 5.62 |
| CHEMBL4743495 | — | Ki | = | 2537.17 | nM | 5.60 |
| CHEMBL2435402 | — | Ki | = | 3259.49 | nM | 5.49 |
| CHEMBL4743049 | — | Ki | = | 7437.04 | nM | 5.13 |
| CHEMBL3215877 | — | Ki | = | 7498.94 | nM | 5.12 |