Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5442190

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
PDSP Secondary Binding target: OPRM1 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Category PDSP assay
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL233)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

EUbOPEN Chemogenomics Library - PDSP Secondary Binding
EUbOPEN
(2024)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 33 6.41 8.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1707 LOPERAMIDE HYDROCHLORIDE 4.0 1 8.59
CHEMBL1255661 1 8.03
CHEMBL300555 SPIROXATRINE 2.0 1 7.89
CHEMBL25230 1 7.83
CHEMBL445102 1 7.29
CHEMBL1474387 1 6.99
CHEMBL405355 NIGULDIPINE 2.0 1 6.89
CHEMBL5087624 1 6.85
CHEMBL1200517 DIHYDROERGOTAMINE MESYLATE 4.0 1 6.81
CHEMBL79834 SPIRAMIDE 2.0 1 6.81
CHEMBL2138684 FROVATRIPTAN SUCCINATE 4.0 1 6.73
CHEMBL1256844 1 6.73
CHEMBL540612 1 6.60
CHEMBL1256750 1 6.49
CHEMBL513136 1 6.49
CHEMBL267777 RITANSERIN 2.0 1 6.46
CHEMBL344159 TOLVAPTAN 4.0 1 6.39
CHEMBL531742 1 6.30
CHEMBL1615374 VILAZODONE HYDROCHLORIDE 4.0 1 6.14
CHEMBL59898 1 5.95
CHEMBL1200776 CINACALCET HYDROCHLORIDE 4.0 1 5.89
CHEMBL2391908 1 5.89
CHEMBL1558 PRAZOSIN HYDROCHLORIDE 4.0 1 5.75
CHEMBL2107572 TECALCET HYDROCHLORIDE 2.0 1 5.66
CHEMBL1516474 TEGASEROD MALEATE 4.0 1 5.55
CHEMBL259656 1 5.50
CHEMBL542139 1 5.45
CHEMBL1766103 1 5.25
CHEMBL2440454 1 5.20
CHEMBL1376064 1 5.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1707 LOPERAMIDE HYDROCHLORIDE Ki = 2.587 nM 8.59
CHEMBL1255661 Ki = 9.376 nM 8.03
CHEMBL300555 SPIROXATRINE Ki = 12.81 nM 7.89
CHEMBL25230 Ki = 14.68 nM 7.83
CHEMBL445102 Ki = 51.53 nM 7.29
CHEMBL1474387 Ki = 102.35 nM 6.99
CHEMBL405355 NIGULDIPINE Ki = 128.53 nM 6.89
CHEMBL5087624 Ki = 142.4 nM 6.85
CHEMBL1200517 DIHYDROERGOTAMINE MESYLATE Ki = 154.01 nM 6.81
CHEMBL79834 SPIRAMIDE Ki = 155.54 nM 6.81
CHEMBL2138684 FROVATRIPTAN SUCCINATE Ki = 186.98 nM 6.73
CHEMBL1256844 Ki = 184.71 nM 6.73
CHEMBL540612 Ki = 249.03 nM 6.60
CHEMBL1256750 Ki = 323.0 nM 6.49
CHEMBL513136 Ki = 321.0 nM 6.49
CHEMBL267777 RITANSERIN Ki = 344.27 nM 6.46
CHEMBL344159 TOLVAPTAN Ki = 410.01 nM 6.39
CHEMBL531742 Ki = 494.94 nM 6.30
CHEMBL1615374 VILAZODONE HYDROCHLORIDE Ki = 729.62 nM 6.14
CHEMBL59898 Ki = 1130.71 nM 5.95
CHEMBL1200776 CINACALCET HYDROCHLORIDE Ki = 1295.69 nM 5.89
CHEMBL2391908 Ki = 1280.71 nM 5.89
CHEMBL1558 PRAZOSIN HYDROCHLORIDE Ki = 1791.02 nM 5.75
CHEMBL2107572 TECALCET HYDROCHLORIDE Ki = 2205.47 nM 5.66
CHEMBL1516474 TEGASEROD MALEATE Ki = 2846.43 nM 5.55
CHEMBL259656 Ki = 3141.95 nM 5.50
CHEMBL542139 Ki = 3579.73 nM 5.45
CHEMBL1766103 Ki = 5565.45 nM 5.25
CHEMBL2440454 Ki = 6269.02 nM 5.20
CHEMBL1376064 Ki = 7852.36 nM 5.11
CHEMBL3675743 Ki = 7723.25 nM 5.11
CHEMBL2392141 Ki > 10000.0 nM -
CHEMBL494502 Ki > 10000.0 nM -