Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5442205

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
PDSP Secondary Binding target: HTR2A - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
45
Total Activities
45
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Category PDSP assay
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2A (CHEMBL224)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

EUbOPEN Chemogenomics Library - PDSP Secondary Binding
EUbOPEN
(2024)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 45 6.48 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL74355 VOLINANSERIN 3.0 1 9.10
CHEMBL1215661 PRUVANSERIN 2.0 1 8.58
CHEMBL267777 RITANSERIN 2.0 1 8.38
CHEMBL83894 FANANSERIN 2.0 1 8.32
CHEMBL1489369 1 7.92
CHEMBL79834 SPIRAMIDE 2.0 1 7.82
CHEMBL1200492 NEFAZODONE HYDROCHLORIDE 4.0 1 7.81
CHEMBL543113 1 7.76
CHEMBL2105760 BREXPIPRAZOLE 4.0 1 7.74
CHEMBL1200517 DIHYDROERGOTAMINE MESYLATE 4.0 1 7.68
CHEMBL5089005 1 7.45
CHEMBL1633 KETOTIFEN FUMARATE 4.0 1 7.36
CHEMBL2024517 CARIPRAZINE HYDROCHLORIDE 4.0 1 7.21
CHEMBL4743049 1 6.98
CHEMBL5075931 1 6.96
CHEMBL2107572 TECALCET HYDROCHLORIDE 2.0 1 6.74
CHEMBL103769 1 6.69
CHEMBL1200809 AZELASTINE HYDROCHLORIDE 4.0 1 6.64
CHEMBL1516474 TEGASEROD MALEATE 4.0 1 6.37
CHEMBL531742 1 6.26
CHEMBL300555 SPIROXATRINE 2.0 1 6.15
CHEMBL589390 LATREPIRDINE 3.0 1 6.14
CHEMBL1200776 CINACALCET HYDROCHLORIDE 4.0 1 6.08
CHEMBL445102 1 6.05
CHEMBL1256998 1 6.02
CHEMBL3716980 1 6.01
CHEMBL1263 SALMETEROL 4.0 1 5.89
CHEMBL567303 PALOSURAN 2.0 1 5.80
CHEMBL1671 PROPRANOLOL HYDROCHLORIDE 4.0 1 5.75
CHEMBL281350 SB-224289 1 5.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL74355 VOLINANSERIN Ki = 0.7877 nM 9.10
CHEMBL1215661 PRUVANSERIN Ki = 2.638 nM 8.58
CHEMBL267777 RITANSERIN Ki = 4.221 nM 8.38
CHEMBL83894 FANANSERIN Ki = 4.784 nM 8.32
CHEMBL1489369 Ki = 11.89 nM 7.92
CHEMBL79834 SPIRAMIDE Ki = 15.05 nM 7.82
CHEMBL1200492 NEFAZODONE HYDROCHLORIDE Ki = 15.62 nM 7.81
CHEMBL543113 Ki = 17.38 nM 7.76
CHEMBL2105760 BREXPIPRAZOLE Ki = 18.26 nM 7.74
CHEMBL1200517 DIHYDROERGOTAMINE MESYLATE Ki = 21.05 nM 7.68
CHEMBL5089005 Ki = 35.74 nM 7.45
CHEMBL1633 KETOTIFEN FUMARATE Ki = 43.93 nM 7.36
CHEMBL2024517 CARIPRAZINE HYDROCHLORIDE Ki = 61.19 nM 7.21
CHEMBL4743049 Ki = 104.86 nM 6.98
CHEMBL5075931 Ki = 111.0 nM 6.96
CHEMBL2107572 TECALCET HYDROCHLORIDE Ki = 180.96 nM 6.74
CHEMBL103769 Ki = 202.3 nM 6.69
CHEMBL1200809 AZELASTINE HYDROCHLORIDE Ki = 229.61 nM 6.64
CHEMBL1516474 TEGASEROD MALEATE Ki = 431.42 nM 6.37
CHEMBL531742 Ki = 548.24 nM 6.26
CHEMBL300555 SPIROXATRINE Ki = 702.1 nM 6.15
CHEMBL589390 LATREPIRDINE Ki = 725.83 nM 6.14
CHEMBL1200776 CINACALCET HYDROCHLORIDE Ki = 841.68 nM 6.08
CHEMBL445102 Ki = 898.67 nM 6.05
CHEMBL1256998 Ki = 963.6 nM 6.02
CHEMBL3716980 Ki = 982.99 nM 6.01
CHEMBL1263 SALMETEROL Ki = 1274.77 nM 5.89
CHEMBL567303 PALOSURAN Ki = 1588.55 nM 5.80
CHEMBL1671 PROPRANOLOL HYDROCHLORIDE Ki = 1790.2 nM 5.75
CHEMBL281350 SB-224289 Ki = 1784.23 nM 5.75
CHEMBL1095777 INDACATEROL Ki = 2019.53 nM 5.70
CHEMBL3342358 Ki = 2119.58 nM 5.67
CHEMBL9387 L-365260 Ki = 2140.92 nM 5.67
CHEMBL3215877 Ki = 2290.34 nM 5.64
CHEMBL259656 Ki = 2657.36 nM 5.58
CHEMBL1474387 Ki = 2787.73 nM 5.55
CHEMBL1766103 Ki = 3429.25 nM 5.46
CHEMBL494502 Ki = 3878.82 nM 5.41
CHEMBL3338866 MK-1064 Ki = 4592.51 nM 5.34
CHEMBL3741589 Ki = 4606.27 nM 5.34
CHEMBL519240 Ki = 6425.4 nM 5.19
CHEMBL260563 Ki = 7872.27 nM 5.10
CHEMBL1705 CLONIDINE HYDROCHLORIDE Ki = 9141.13 nM 5.04
CHEMBL1593850 Ki = 9751.39 nM 5.01
CHEMBL502882 Ki > 10000.0 nM -