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Assay Detail

CHEMBL5615906

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human URAT1 transfected in HEK293 cells by measuring 14C-uric acid uptake preincubated for 30 mins followed by 14--uric acid addition and measured after 15 mins by liquid scintillation counter analysis
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Solute carrier family 22 member 12 (CHEMBL6120)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and bioactivity evaluation of isobavachin derivatives as hURAT1 inhibitors for hyperuricemia agents.
Zhao Z, Chen X, Luo J, Chen M, Luo J, Chen J, Li Z, Wan S, Wu T, Zhang J, Pang J, Tian Y.
Eur J Med Chem (2024) 277 : 116753 -116753
PubMed 39142150 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 5.91 6.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5618375 1 6.80
CHEMBL5620069 1 6.72
CHEMBL5618313 1 6.64
CHEMBL5618175 1 6.62
CHEMBL5618500 1 6.44
CHEMBL5620318 1 6.19
CHEMBL5620253 1 6.19
CHEMBL5618811 1 6.14
CHEMBL5619569 1 6.08
CHEMBL5618194 1 6.07
CHEMBL5619241 1 6.02
CHEMBL5619047 1 6.01
CHEMBL5620313 1 5.79
CHEMBL5619255 1 5.72
CHEMBL5620074 1 5.68
CHEMBL5618197 1 5.66
CHEMBL5620142 1 5.66
CHEMBL5619092 1 5.62
CHEMBL5620285 1 5.60
CHEMBL5618751 1 5.57
CHEMBL5619622 1 5.54
CHEMBL5618630 1 5.52
CHEMBL5618105 1 5.45
CHEMBL5619161 1 5.37
CHEMBL5619991 1 5.35
CHEMBL2105720 LESINURAD 4.0 1 5.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5618375 IC50 = 160.0 nM 6.80
CHEMBL5620069 IC50 = 190.0 nM 6.72
CHEMBL5618313 IC50 = 230.0 nM 6.64
CHEMBL5618175 IC50 = 240.0 nM 6.62
CHEMBL5618500 IC50 = 360.0 nM 6.44
CHEMBL5620318 IC50 = 650.0 nM 6.19
CHEMBL5620253 IC50 = 650.0 nM 6.19
CHEMBL5618811 IC50 = 730.0 nM 6.14
CHEMBL5619569 IC50 = 830.0 nM 6.08
CHEMBL5618194 IC50 = 860.0 nM 6.07
CHEMBL5619241 IC50 = 950.0 nM 6.02
CHEMBL5619047 IC50 = 980.0 nM 6.01
CHEMBL5620313 IC50 = 1640.0 nM 5.79
CHEMBL5619255 IC50 = 1890.0 nM 5.72
CHEMBL5620074 IC50 = 2110.0 nM 5.68
CHEMBL5618197 IC50 = 2180.0 nM 5.66
CHEMBL5620142 IC50 = 2180.0 nM 5.66
CHEMBL5619092 IC50 = 2410.0 nM 5.62
CHEMBL5620285 IC50 = 2500.0 nM 5.60
CHEMBL5618751 IC50 = 2680.0 nM 5.57
CHEMBL5619622 IC50 = 2860.0 nM 5.54
CHEMBL5618630 IC50 = 3020.0 nM 5.52
CHEMBL5618105 IC50 = 3590.0 nM 5.45
CHEMBL5619161 IC50 = 4240.0 nM 5.37
CHEMBL5619991 IC50 = 4430.0 nM 5.35
CHEMBL2105720 LESINURAD IC50 = 7180.0 nM 5.14