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Assay Detail

CHEMBL632853

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Ability to enhance the binding of (+/-)-[3H]nicotine to the rat brain P2 fraction at binding site 2
32
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Neuronal acetylcholine receptor (CHEMBL2094110)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Structure-activity relationships of some pyridine, piperidine, and pyrrolidine analogues for enhancing and inhibiting the binding of (+/-)-[3H]nicotine to the rat brain P2 preparation.
Sloan JW, Martin WR, Hook R, Hernandez J.
J Med Chem (1985) 28 : 1245 -1251
PubMed 4032427 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 32 5.87 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3 NICOTINE 4.0 2 11.00
CHEMBL15732 1 7.30
CHEMBL15487 PIPERIDINE -1.0 1 6.92
CHEMBL21824 1 5.77
CHEMBL25053 1 5.48
CHEMBL23025 1 5.02
CHEMBL22830 PYRROLIDINE 3 4.60
CHEMBL665 N-METHYL PYRROLIDINE (NMP) 1 4.28
CHEMBL22977 1 4.06
CHEMBL21867 1 4.01
CHEMBL279305 1 4.00
CHEMBL662 1 -
CHEMBL284022 1 -
CHEMBL279512 1 -
CHEMBL22463 1 -
CHEMBL22976 1 -
CHEMBL25541 1 -
CHEMBL277887 1 -
CHEMBL266158 PYRIDINE 1 -
CHEMBL21619 2-AMINOPYRIDINE 1 -
CHEMBL664 1 -
CHEMBL21531 1 -
CHEMBL281417 N-METHYLPIPERIDINE 1 -
CHEMBL21533 1 -
CHEMBL21454 1 -
CHEMBL22270 1 -
CHEMBL22332 1 -
CHEMBL280963 DL-ANABASINE 1 -
CHEMBL15544 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3 NICOTINE Kd = 0.01 nM 11.00
CHEMBL3 NICOTINE Kd = 10.0 nM 8.00
CHEMBL15732 Kd = 50.0 nM 7.30
CHEMBL15487 PIPERIDINE Kd = 120.0 nM 6.92
CHEMBL21824 Kd = 1700.0 nM 5.77
CHEMBL25053 Kd = 3300.0 nM 5.48
CHEMBL23025 Kd = 9500.0 nM 5.02
CHEMBL22830 PYRROLIDINE Kd = 25000.0 nM 4.60
CHEMBL665 N-METHYL PYRROLIDINE (NMP) Kd = 52000.0 nM 4.28
CHEMBL22977 Kd = 87000.0 nM 4.06
CHEMBL21867 Kd = 97000.0 nM 4.01
CHEMBL279305 Kd = 100000.0 nM 4.00
CHEMBL21619 2-AMINOPYRIDINE Kd = 200000.0 nM -
CHEMBL25541 Kd = 380000.0 nM -
CHEMBL22463 Kd = 820000.0 nM -
CHEMBL284022 Kd = 3000000.0 nM -
CHEMBL664 Kd = 4700000.0 nM -
CHEMBL662 Kd = 880000.0 nM -
CHEMBL279512 Kd = 890000.0 nM -
CHEMBL22976 Kd = 10000000.0 nM -
CHEMBL22830 PYRROLIDINE Kd = 330000.0 nM -
CHEMBL22830 PYRROLIDINE Kd = 41000000.0 nM -
CHEMBL277887 Kd = 6000000.0 nM -
CHEMBL266158 PYRIDINE Kd = 3300000.0 nM -
CHEMBL22332 Kd = 3400000.0 nM -
CHEMBL22270 Kd = 7900000.0 nM -
CHEMBL21454 Kd = 3200000.0 nM -
CHEMBL281417 N-METHYLPIPERIDINE Kd = 1200000.0 nM -
CHEMBL21531 Kd = 2700000.0 nM -
CHEMBL15544 Kd = 2200000.0 nM -
CHEMBL280963 DL-ANABASINE Kd = 1300000.0 nM -
CHEMBL21533 Kd = 3900000.0 nM -