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Assay Detail

CHEMBL681469

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Concentration required to displace 50% of a potent radiolabeled FTI from farnesyl transferase in cultured H-ras-transformed Rat1 cells
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type Rat1
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

Protein farnesyltransferase (CHEMBL2094108)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Design and biological activity of (S)-4-(5-([1-(3-chlorobenzyl)-2-oxopyrrolidin-3-ylamino]methyl)imidazol-1-ylmethyl)benzonitrile, a 3-aminopyrrolidinone farnesyltransferase inhibitor with excellent cell potency.
Bell IM, Gallicchio SN, Abrams M, Beshore DC, Buser CA, Culberson JC, Davide J, Ellis-Hutchings M, Fernandes C, Gibbs JB, Graham SL, Hartman GD, Heimbrook DC, Homnick CF, Huff JR, Kassahun K, Koblan KS, Kohl NE, Lobell RB, Lynch JJ, Miller PA, Omer CA, Rodrigues AD, Walsh ES, Williams TM.
J Med Chem (2001) 44 : 2933 -2949
PubMed 11520202 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 7.36 9.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL312137 1 9.28
CHEMBL100661 1 8.96
CHEMBL318438 1 8.57
CHEMBL100798 1 8.48
CHEMBL316502 1 8.24
CHEMBL98361 1 8.21
CHEMBL100566 1 8.02
CHEMBL316726 1 7.92
CHEMBL99381 1 7.85
CHEMBL100579 1 7.68
CHEMBL97925 1 7.57
CHEMBL101561 1 7.52
CHEMBL99205 1 7.52
CHEMBL329732 1 7.44
CHEMBL100415 1 7.38
CHEMBL98974 1 7.31
CHEMBL327500 1 7.28
CHEMBL98553 1 7.19
CHEMBL317520 1 7.17
CHEMBL101467 1 7.16
CHEMBL329067 1 7.13
CHEMBL327946 1 6.96
CHEMBL319737 1 6.92
CHEMBL98147 1 6.82
CHEMBL418982 1 6.82
CHEMBL99371 1 6.70
CHEMBL99435 1 6.62
CHEMBL101788 1 6.52
CHEMBL98711 1 6.51
CHEMBL327278 1 6.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL312137 IC50 = 0.52 nM 9.28
CHEMBL100661 IC50 = 1.1 nM 8.96
CHEMBL318438 IC50 = 2.7 nM 8.57
CHEMBL100798 IC50 = 3.3 nM 8.48
CHEMBL316502 IC50 = 5.7 nM 8.24
CHEMBL98361 IC50 = 6.2 nM 8.21
CHEMBL100566 IC50 = 9.5 nM 8.02
CHEMBL316726 IC50 = 12.0 nM 7.92
CHEMBL99381 IC50 = 14.0 nM 7.85
CHEMBL100579 IC50 = 21.0 nM 7.68
CHEMBL97925 IC50 = 27.0 nM 7.57
CHEMBL101561 IC50 = 30.0 nM 7.52
CHEMBL99205 IC50 = 30.0 nM 7.52
CHEMBL329732 IC50 = 36.0 nM 7.44
CHEMBL100415 IC50 = 42.0 nM 7.38
CHEMBL98974 IC50 = 49.0 nM 7.31
CHEMBL327500 IC50 = 53.0 nM 7.28
CHEMBL98553 IC50 = 65.0 nM 7.19
CHEMBL317520 IC50 = 67.0 nM 7.17
CHEMBL101467 IC50 = 70.0 nM 7.16
CHEMBL329067 IC50 = 74.0 nM 7.13
CHEMBL327946 IC50 = 110.0 nM 6.96
CHEMBL319737 IC50 = 120.0 nM 6.92
CHEMBL98147 IC50 = 150.0 nM 6.82
CHEMBL418982 IC50 = 150.0 nM 6.82
CHEMBL99371 IC50 = 200.0 nM 6.70
CHEMBL99435 IC50 = 240.0 nM 6.62
CHEMBL101788 IC50 = 300.0 nM 6.52
CHEMBL98711 IC50 = 310.0 nM 6.51
CHEMBL327278 IC50 = 330.0 nM 6.48
CHEMBL318943 IC50 = 460.0 nM 6.34
CHEMBL317129 IC50 = 610.0 nM 6.21
CHEMBL322007 IC50 = 1100.0 nM 5.96