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Assay Detail

CHEMBL701547

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibition of the specific binding of [3H]AMPA to Ionotropic glutamate receptor AMPA in rat whole brain membranes in the presence of 100 uM KSCN
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Subcellular Brain membranes
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Glutamate receptor ionotropic, AMPA (CHEMBL2093871)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Novel alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate receptor antagonists: synthesis and structure-activity relationships of 6-(1H-imidazol-1-yl)-7-nitro-2,3(1H,4H)-pyrido[2,3-b]pyrazinedione and related compounds.
Ohmori J, Kubota H, Shimizu-Sasamata M, Okada M, Sakamoto S.
J Med Chem (1996) 39 : 1331 -1338
PubMed 8632440 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 6.21 7.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL13260 YM-90K 1 7.08
CHEMBL27653 1 6.85
CHEMBL25922 1 6.16
CHEMBL26699 1 6.07
CHEMBL282417 1 6.04
CHEMBL286752 1 5.96
CHEMBL27715 1 5.80
CHEMBL23044 1 5.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL13260 YM-90K Ki = 84.0 nM 7.08
CHEMBL27653 Ki = 140.0 nM 6.85
CHEMBL25922 Ki = 700.0 nM 6.16
CHEMBL26699 Ki = 860.0 nM 6.07
CHEMBL282417 Ki = 920.0 nM 6.04
CHEMBL286752 Ki = 1100.0 nM 5.96
CHEMBL27715 Ki = 1600.0 nM 5.80
CHEMBL23044 Ki = 2000.0 nM 5.70