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Assay Detail

CHEMBL875242

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]clonidine from adrenergic alpha 2 receptor of rat cerebral cortical membranes
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Adrenergic receptor alpha-2 (CHEMBL2093864)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

Syntheses of 2,5- and 2,6-difluoronorepinephrine, 2,5-difluoroepinephrine, and 2,6-difluorophenylephrine: effect of disubstitution with fluorine on adrenergic activity.
Chen GT, King M, Gusovsky F, Creveling CR, Daly JW, Chen BH, Nie JY, Kirk KL.
J Med Chem (1993) 36 : 3947 -3955
PubMed 8254623 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 6.58 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL42359 1 8.05
CHEMBL1437 NOREPINEPHRINE 4.0 1 7.42
CHEMBL679 EPINEPHRINE 4.0 1 7.42
CHEMBL43711 1 7.18
CHEMBL281232 1 7.10
CHEMBL129190 1 6.82
CHEMBL61372 1 6.64
CHEMBL1215 PHENYLEPHRINE 4.0 1 6.41
CHEMBL435802 1 6.12
CHEMBL340189 1 6.03
CHEMBL126347 1 5.92
CHEMBL302451 1 5.92
CHEMBL287587 1 5.68
CHEMBL338564 1 5.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL42359 Ki = 9.0 nM 8.05
CHEMBL1437 NOREPINEPHRINE Ki = 38.0 nM 7.42
CHEMBL679 EPINEPHRINE Ki = 38.0 nM 7.42
CHEMBL43711 Ki = 66.0 nM 7.18
CHEMBL281232 Ki = 80.0 nM 7.10
CHEMBL129190 Ki = 150.0 nM 6.82
CHEMBL61372 Ki = 230.0 nM 6.64
CHEMBL1215 PHENYLEPHRINE Ki = 390.0 nM 6.41
CHEMBL435802 Ki = 750.0 nM 6.12
CHEMBL340189 Ki = 940.0 nM 6.03
CHEMBL126347 Ki = 1200.0 nM 5.92
CHEMBL302451 Ki = 1200.0 nM 5.92
CHEMBL287587 Ki = 2100.0 nM 5.68
CHEMBL338564 Ki = 3500.0 nM 5.46