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Assay Detail

CHEMBL876560

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]dihydroalprenolol from adrenergic beta 1 receptor of rat cerebral cortical membranes
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Expert

Target

Beta-1 adrenergic receptor (CHEMBL3252)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Syntheses of 2,5- and 2,6-difluoronorepinephrine, 2,5-difluoroepinephrine, and 2,6-difluorophenylephrine: effect of disubstitution with fluorine on adrenergic activity.
Chen GT, King M, Gusovsky F, Creveling CR, Daly JW, Chen BH, Nie JY, Kirk KL.
J Med Chem (1993) 36 : 3947 -3955
PubMed 8254623 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 5.23 5.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL679 EPINEPHRINE 4.0 1 5.92
CHEMBL129190 1 5.82
CHEMBL1437 NOREPINEPHRINE 4.0 1 5.80
CHEMBL43711 1 5.75
CHEMBL126347 1 5.64
CHEMBL287587 1 5.60
CHEMBL302451 1 5.40
CHEMBL338564 1 5.25
CHEMBL1215 PHENYLEPHRINE 4.0 1 4.89
CHEMBL281232 1 4.35
CHEMBL42359 1 4.20
CHEMBL340189 1 4.09
CHEMBL61372 1 -
CHEMBL435802 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL679 EPINEPHRINE Ki = 1200.0 nM 5.92
CHEMBL129190 Ki = 1500.0 nM 5.82
CHEMBL1437 NOREPINEPHRINE Ki = 1600.0 nM 5.80
CHEMBL43711 Ki = 1800.0 nM 5.75
CHEMBL126347 Ki = 2300.0 nM 5.64
CHEMBL287587 Ki = 2500.0 nM 5.60
CHEMBL302451 Ki = 4000.0 nM 5.40
CHEMBL338564 Ki = 5600.0 nM 5.25
CHEMBL1215 PHENYLEPHRINE Ki = 13000.0 nM 4.89
CHEMBL281232 Ki = 45000.0 nM 4.35
CHEMBL42359 Ki = 63000.0 nM 4.20
CHEMBL340189 Ki = 81000.0 nM 4.09
CHEMBL61372 Ki = 180000.0 nM -
CHEMBL435802 Ki > 50000.0 nM -