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Assay Detail

CHEMBL4402768

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]CGP12177 from human beta2 adrenoceptor expressed in CHO cell membranes
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-2 adrenergic receptor (CHEMBL210)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of covalent antagonists for β1- and β2-adrenergic receptors.
Schwalbe T, Huebner H, Gmeiner P.
Bioorg Med Chem (2019) 27 : 2959 -2971
PubMed 31151791 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 16 7.37 10.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4456301 1 10.14
CHEMBL4537221 1 9.80
CHEMBL27 PROPRANOLOL 4.0 1 9.49
CHEMBL4463925 1 8.96
CHEMBL4463246 1 8.47
CHEMBL4566666 1 7.70
CHEMBL4437569 1 7.29
CHEMBL4553747 1 7.24
CHEMBL13 METOPROLOL 4.0 1 6.66
CHEMBL645 BISOPROLOL 4.0 1 6.60
CHEMBL4473272 1 6.50
CHEMBL280822 CGP-20712A 1 6.00
CHEMBL4456505 1 5.89
CHEMBL4576034 1 5.85
CHEMBL4443911 1 5.70
CHEMBL4450085 1 5.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4456301 Ki = 0.073 nM 10.14
CHEMBL4537221 Ki = 0.16 nM 9.80
CHEMBL27 PROPRANOLOL Ki = 0.32 nM 9.49
CHEMBL4463925 Ki = 1.1 nM 8.96
CHEMBL4463246 Ki = 3.4 nM 8.47
CHEMBL4566666 Ki = 20.0 nM 7.70
CHEMBL4437569 Ki = 51.0 nM 7.29
CHEMBL4553747 Ki = 57.0 nM 7.24
CHEMBL13 METOPROLOL Ki = 220.0 nM 6.66
CHEMBL645 BISOPROLOL Ki = 250.0 nM 6.60
CHEMBL4473272 Ki = 320.0 nM 6.50
CHEMBL280822 CGP-20712A Ki = 1000.0 nM 6.00
CHEMBL4456505 Ki = 1300.0 nM 5.89
CHEMBL4576034 Ki = 1400.0 nM 5.85
CHEMBL4443911 Ki = 2000.0 nM 5.70
CHEMBL4450085 Ki = 2200.0 nM 5.66