Assay Detail
Binding
CHEMBL4880048
Review assay metadata, readout intent, target linkage, and publication context from the same page.
GPCRScan assay: inhibition of H3
195
Total Activities
181
Compounds Tested
2
Activity Types
12
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
SGC PDSP GPCRScan assays
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Inhibition | 181 | - | - |
| Ki | 14 | 5.92 | 7.09 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Parental cells | Parental cells | - | — | HEK Flp-In |
| PMID | PMID | - | — | 1.2706455E7 |
| Protein length | Protein length | - | — | full-length |
| Radioligand conc. used [nM] | Radioligand conc. used [nM] | - | — | 0.5 – 1.0 |
| Radioligand Kd | Radioligand Kd | 0.78 | nM | — |
| Radioligand name | Radioligand name | - | — | [3H]-alpha-methylhistamine |
| Radioligand pKd | Radioligand pKd | 9.11 | — | — |
| Radioligand pKd ± SEM | Radioligand pKd ± SEM | 0.07 | — | — |
| Reference ligand | Reference ligand | - | — | Histamine |
| Reference ligand Ki | Reference ligand Ki | 4.99 | nM | — |
| Reference ligand pKi | Reference ligand pKi | 8.3 | — | — |
| Reference ligand pKi ± SEM | Reference ligand pKi ± SEM | 0.03 | — | — |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4748908 | TP-040n | — | 5 | 7.09 |
| CHEMBL3342332 | A-1155463 | — | 3 | 5.74 |
| CHEMBL4445645 | — | — | 3 | 5.69 |
| CHEMBL3617968 | BAY-678 | — | 3 | 5.55 |
| CHEMBL3685796 | MRL-SYKi | — | 3 | 5.00 |
| CHEMBL3480577 | UCSF924 | — | 3 | 5.00 |
| CHEMBL4539943 | BAY-694 | — | 1 | - |
| CHEMBL3907057 | BAY-826 | — | 1 | - |
| CHEMBL4521594 | BAY-1797 | — | 1 | - |
| CHEMBL4516843 | BAY-207 | — | 1 | - |
| CHEMBL4537788 | BAY-784 | — | 1 | - |
| CHEMBL4564312 | BAY-786 | — | 1 | - |
| CHEMBL4448899 | BAY-876 | — | 1 | - |
| CHEMBL4552180 | BAY-588 | — | 1 | - |
| CHEMBL4445670 | BAY-885 | — | 1 | - |
| CHEMBL4455582 | BAY-693 | — | 1 | - |
| CHEMBL4630644 | BAY-386 | — | 1 | - |
| CHEMBL4630643 | BAY-448 | — | 1 | - |
| CHEMBL4585243 | BAY-7598 | — | 1 | - |
| CHEMBL4522773 | BAY-827 | — | 1 | - |
| CHEMBL4593677 | BAY-474 | — | 1 | - |
| CHEMBL4066040 | A-1596584 | — | 1 | - |
| CHEMBL4093612 | A-1596586 | — | 1 | - |
| CHEMBL332794 | A-192621 | — | 1 | - |
| CHEMBL4513786 | A-545 | — | 1 | - |
| CHEMBL111612 | ABT-546 | — | 1 | - |
| CHEMBL4526341 | A-108 | — | 1 | - |
| CHEMBL4586794 | ABT-100 | — | 1 | - |
| CHEMBL3701197 | A-226 | — | 1 | - |
| CHEMBL3697701 | A-079 | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4748908 | TP-040n | Ki | = | 81.28 | nM | 7.09 |
| CHEMBL4748908 | TP-040n | Ki | = | 93.33 | nM | 7.03 |
| CHEMBL4748908 | TP-040n | Ki | = | 92.76 | nM | 7.03 |
| CHEMBL4748908 | TP-040n | Ki | = | 99.57 | nM | 7.00 |
| CHEMBL3342332 | A-1155463 | Ki | = | 1819.7 | nM | 5.74 |
| CHEMBL3342332 | A-1155463 | Ki | = | 1806.76 | nM | 5.74 |
| CHEMBL4445645 | — | Ki | = | 2041.74 | nM | 5.69 |
| CHEMBL4445645 | — | Ki | = | 2333.56 | nM | 5.63 |
| CHEMBL3617968 | BAY-678 | Ki | = | 2818.38 | nM | 5.55 |
| CHEMBL3617968 | BAY-678 | Ki | = | 3068.5 | nM | 5.51 |
| CHEMBL3685796 | MRL-SYKi | Ki | = | 10000.0 | nM | 5.00 |
| CHEMBL3480577 | UCSF924 | Ki | = | 10000.0 | nM | 5.00 |
| CHEMBL3685796 | MRL-SYKi | Ki | = | 10000.0 | nM | 5.00 |
| CHEMBL4075278 | — | Inhibition | = | 15.08 | % | - |
| CHEMBL4514203 | BAY-390 | Inhibition | = | -12.85 | % | - |
| CHEMBL4647642 | BAY-173 | Inhibition | = | 9.39 | % | - |
| CHEMBL4460070 | ZINC32547799 | Inhibition | = | 6.14 | % | - |
| CHEMBL5027912 | — | Inhibition | = | 7.29 | % | - |
| CHEMBL5092747 | — | Inhibition | = | 6.04 | % | - |
| CHEMBL5094181 | — | Inhibition | = | -7.55 | % | - |
| CHEMBL4592872 | BAY-897 | Inhibition | = | 3.57 | % | - |
| CHEMBL4537673 | BAY-985 | Inhibition | = | -3.83 | % | - |
| CHEMBL4538591 | BAY-440 | Inhibition | = | 4.68 | % | - |
| CHEMBL4580220 | BAY-3827 | Inhibition | = | -10.83 | % | - |
| CHEMBL4630642 | BAY-974 | Inhibition | = | 1.89 | % | - |
| CHEMBL4456585 | WM-1119 | Inhibition | = | -20.48 | % | - |
| CHEMBL4520293 | (R)-ZINC-3573 | Inhibition | = | 38.64 | % | - |
| CHEMBL4534980 | (S)-ZINC-3573 | Inhibition | = | 13.43 | % | - |
| CHEMBL4635540 | GSK791 | Inhibition | = | -2.09 | % | - |
| CHEMBL4555711 | BAY-9897 | Inhibition | = | -2.34 | % | - |
| CHEMBL4648362 | GSK789 | Inhibition | = | -9.93 | % | - |
| CHEMBL4638939 | GSK943 | Inhibition | = | -5.65 | % | - |
| CHEMBL4635134 | GSK620 | Inhibition | = | -8.16 | % | - |
| CHEMBL4518706 | PF-00911705 | Inhibition | = | 12.94 | % | - |
| CHEMBL3137331 | DEFACTINIB | Inhibition | = | -7.94 | % | - |
| CHEMBL4577237 | BI 9446 | Inhibition | = | 19.99 | % | - |
| CHEMBL4530740 | BI 1950 | Inhibition | = | 1.43 | % | - |
| CHEMBL4552554 | PF-04475866 | Inhibition | = | -19.98 | % | - |
| CHEMBL3286797 | PF-04418948 | Inhibition | = | -15.34 | % | - |
| CHEMBL2204283 | MSD-M1PAM-NC | Inhibition | = | 11.22 | % | - |
| CHEMBL1800285 | MSD-M1PAM | Inhibition | = | 4.08 | % | - |
| CHEMBL4063249 | TP-021n | Inhibition | = | 8.47 | % | - |
| CHEMBL4637208 | GSK973 | Inhibition | = | -9.57 | % | - |
| CHEMBL4639128 | GSK778 | Inhibition | = | -5.03 | % | - |
| CHEMBL4545807 | BAY-070 | Inhibition | = | -7.57 | % | - |
| CHEMBL4798829 | — | Inhibition | = | -4.94 | % | - |
| CHEMBL4536197 | A-486 | Inhibition | = | 13.21 | % | - |
| CHEMBL4282264 | A-485 | Inhibition | = | 0.39 | % | - |
| CHEMBL4561806 | BTZO-4 | Inhibition | = | 8.097 | % | - |
| CHEMBL4516611 | BTZO-1 | Inhibition | = | 1.999 | % | - |