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Assay Detail

CHEMBL4880060

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Binding
GPCRScan assay: inhibition of M4
185
Total Activities
181
Compounds Tested
2
Activity Types
12
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M4 (CHEMBL1821)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

SGC PDSP GPCRScan assays
SGC Frankfurt
(2021)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Inhibition 181 - -
Ki 4 6.21 6.54

Assay Parameters

Parameter Type Value Units Text
Parental cells Parental cells - stable CHO
PMID PMID - 3443095/11578621
Protein length Protein length - full-length
Radioligand conc. used [nM] Radioligand conc. used [nM] - 0.3 – 1.0
Radioligand Kd Radioligand Kd 0.23 nM
Radioligand name Radioligand name - [3H]-QNB
Radioligand pKd Radioligand pKd 9.64
Radioligand pKd ± SEM Radioligand pKd ± SEM 0.06
Reference ligand Reference ligand - Atropine
Reference ligand Ki Reference ligand Ki 0.56 nM
Reference ligand pKi Reference ligand pKi 9.25
Reference ligand pKi ± SEM Reference ligand pKi ± SEM 0.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4520788 BAY-294 3 6.54
CHEMBL5092747 3 5.88
CHEMBL3617968 BAY-678 1 -
CHEMBL4084419 1 -
CHEMBL4096813 1 -
CHEMBL4526351 BAY-309 1 -
CHEMBL3907057 BAY-826 1 -
CHEMBL4630643 BAY-448 1 -
CHEMBL4630644 BAY-386 1 -
CHEMBL4455582 BAY-693 1 -
CHEMBL4445670 BAY-885 1 -
CHEMBL4552180 BAY-588 1 -
CHEMBL4448899 BAY-876 1 -
CHEMBL4564312 BAY-786 1 -
CHEMBL4537788 BAY-784 1 -
CHEMBL4516843 BAY-207 1 -
CHEMBL4521594 BAY-1797 1 -
CHEMBL4539943 BAY-694 1 -
CHEMBL4585243 BAY-7598 1 -
CHEMBL4522773 BAY-827 1 -
CHEMBL4593677 BAY-474 1 -
CHEMBL4066040 A-1596584 1 -
CHEMBL4093612 A-1596586 1 -
CHEMBL332794 A-192621 1 -
CHEMBL4513786 A-545 1 -
CHEMBL111612 ABT-546 1 -
CHEMBL4526341 A-108 1 -
CHEMBL4586794 ABT-100 1 -
CHEMBL3701197 A-226 1 -
CHEMBL3697701 A-079 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4520788 BAY-294 Ki = 291.74 nM 6.54
CHEMBL4520788 BAY-294 Ki = 303.26 nM 6.52
CHEMBL5092747 Ki = 1318.26 nM 5.88
CHEMBL5092747 Ki = 1307.68 nM 5.88
CHEMBL4638406 BAY-3153 Inhibition = -3.29 % -
CHEMBL5306285 Inhibition = 7.09 % -
CHEMBL4647642 BAY-173 Inhibition = -6.12 % -
CHEMBL4075278 Inhibition = 48.66 % -
CHEMBL4063249 TP-021n Inhibition = 11.56 % -
CHEMBL4516611 BTZO-1 Inhibition = -11.99 % -
CHEMBL4561806 BTZO-4 Inhibition = -4.702 % -
CHEMBL4282264 A-485 Inhibition = 11.19 % -
CHEMBL4536197 A-486 Inhibition = 30.37 % -
CHEMBL4798829 Inhibition = -4.61 % -
CHEMBL4545807 BAY-070 Inhibition = 14.7 % -
CHEMBL4639128 GSK778 Inhibition = 15.71 % -
CHEMBL4637208 GSK973 Inhibition = 2.78 % -
CHEMBL4638939 GSK943 Inhibition = 6.19 % -
CHEMBL1800285 MSD-M1PAM Inhibition = -2.36 % -
CHEMBL2204283 MSD-M1PAM-NC Inhibition = -8.87 % -
CHEMBL3286797 PF-04418948 Inhibition = -18.57 % -
CHEMBL4552554 PF-04475866 Inhibition = -15.97 % -
CHEMBL4530740 BI 1950 Inhibition = 12.11 % -
CHEMBL4577237 BI 9446 Inhibition = 21.59 % -
CHEMBL3137331 DEFACTINIB Inhibition = -19.39 % -
CHEMBL4518706 PF-00911705 Inhibition = 8.94 % -
CHEMBL4568735 NVS-MALT1 Inhibition = 21.29 % -
CHEMBL4534980 (S)-ZINC-3573 Inhibition = 34.36 % -
CHEMBL3589752 IPP/CNRS-A017 Inhibition = -16.37 % -
CHEMBL4551344 A-1210227 Inhibition = -5.79 % -
CHEMBL3590047 IPP/CNRS-A019 Inhibition = 10.27 % -
CHEMBL4458424 BAY-899 Inhibition = 22.81 % -
CHEMBL4592872 BAY-897 Inhibition = 9.29 % -
CHEMBL4537673 BAY-985 Inhibition = 24.7 % -
CHEMBL4538591 BAY-440 Inhibition = 4.83 % -
CHEMBL4580220 BAY-3827 Inhibition = 8.27 % -
CHEMBL4630642 BAY-974 Inhibition = 6.87 % -
CHEMBL4456585 WM-1119 Inhibition = 20.45 % -
CHEMBL4520293 (R)-ZINC-3573 Inhibition = 28.79 % -
CHEMBL4635134 GSK620 Inhibition = 0.52 % -
CHEMBL4460070 ZINC32547799 Inhibition = 21.61 % -
CHEMBL4088216 TP-030-1 Inhibition = 8.56 % -
CHEMBL4549667 TP-030-2 Inhibition = 11.74 % -
CHEMBL4551635 BAY-069 Inhibition = -8.67 % -
CHEMBL4643852 BAY-6672 Inhibition = 19.43 % -
CHEMBL4514203 BAY-390 Inhibition = 13.93 % -
CHEMBL4555711 BAY-9897 Inhibition = -3.95 % -
CHEMBL4648362 GSK789 Inhibition = 17.14 % -
CHEMBL4635540 GSK791 Inhibition = -3.0 % -
CHEMBL4636881 GSK046 Inhibition = -0.27 % -