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Assay Detail

CHEMBL651635

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro beta-2 adrenergic receptor activity was determined by measuring inhibition of the isoproterenol induced relaxation in isolated guinea pig tracheal chains contracted with PGF2-alpha
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Beta-2 adrenergic receptor (CHEMBL5414)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Beta 1-selective adrenoceptor antagonists: examples of the 2-[4-[3-(substituted-amino)-2-hydroxypropoxy]phenyl]imidazole class.
Baldwin JJ, Denny GH, Hirschmann R, Freedman MB, Ponticello GS, Gross DM, Sweet CS.
J Med Chem (1983) 26 : 950 -957
PubMed 6134834 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 27 6.10 9.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL499 TIMOLOL 4.0 1 9.62
CHEMBL27 PROPRANOLOL 4.0 1 8.52
CHEMBL3085291 1 8.05
CHEMBL366769 1 7.48
CHEMBL2096752 1 7.24
CHEMBL423 BETAXOLOL 4.0 1 6.98
CHEMBL11171 1 6.88
CHEMBL173424 1 6.84
CHEMBL13 METOPROLOL 4.0 1 6.79
CHEMBL427297 1 6.25
CHEMBL174783 1 6.21
CHEMBL2096750 1 6.06
CHEMBL24 ATENOLOL 4.0 1 5.93
CHEMBL366389 1 5.89
CHEMBL434360 1 5.60
CHEMBL6995 PRACTOLOL 4.0 1 5.35
CHEMBL1788320 1 5.13
CHEMBL177765 1 4.91
CHEMBL366573 1 4.57
CHEMBL2096751 1 4.53
CHEMBL173629 1 4.46
CHEMBL177860 1 4.44
CHEMBL174259 1 4.34
CHEMBL11671 1 4.32
CHEMBL172431 1 -
CHEMBL174106 1 -
CHEMBL2093062 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL499 TIMOLOL Kd = 0.2399 nM 9.62
CHEMBL27 PROPRANOLOL Kd = 3.02 nM 8.52
CHEMBL3085291 Kd = 8.913 nM 8.05
CHEMBL366769 Kd = 33.11 nM 7.48
CHEMBL2096752 Kd = 57.54 nM 7.24
CHEMBL423 BETAXOLOL Kd = 104.71 nM 6.98
CHEMBL11171 Kd = 131.83 nM 6.88
CHEMBL173424 Kd = 144.54 nM 6.84
CHEMBL13 METOPROLOL Kd = 162.18 nM 6.79
CHEMBL427297 Kd = 562.34 nM 6.25
CHEMBL174783 Kd = 616.6 nM 6.21
CHEMBL2096750 Kd = 870.96 nM 6.06
CHEMBL24 ATENOLOL Kd = 1174.9 nM 5.93
CHEMBL366389 Kd = 1288.25 nM 5.89
CHEMBL434360 Kd = 2511.89 nM 5.60
CHEMBL6995 PRACTOLOL Kd = 4466.84 nM 5.35
CHEMBL1788320 Kd = 7413.1 nM 5.13
CHEMBL177765 Kd = 12302.69 nM 4.91
CHEMBL366573 Kd = 26915.35 nM 4.57
CHEMBL2096751 Kd = 29512.09 nM 4.53
CHEMBL173629 Kd = 34673.69 nM 4.46
CHEMBL177860 Kd = 36307.81 nM 4.44
CHEMBL174259 Kd = 45708.82 nM 4.34
CHEMBL11671 Kd = 47863.01 nM 4.32
CHEMBL172431 Kd = 107151.93 nM -
CHEMBL174106 Kd = 186208.71 nM -
CHEMBL2093062 Kd > 316227.77 nM -