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Assay Detail

CHEMBL747405

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Ability to displace [3H]cis--2-methyl-5-((dimethylamino)methyl)-1,3-di oxolane from muscarinic acetylcholine receptor in rat cortical tissue.
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Muscarinic acetylcholine receptor (CHEMBL1907609)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

Muscarinic activity of the thiolactone, lactam, lactol, and thiolactol analogues of pilocarpine and a hypothetical model for the binding of agonists to the m1 receptor.
Shapiro G, Floersheim P, Boelsterli J, Amstutz R, Bolliger G, Gammenthaler H, Gmelin G, Supavilai P, Walkinshaw M.
J Med Chem (1992) 35 : 15 -27
PubMed 1732522 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 6.85 8.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL7634 OXOTREMORINE 1 8.55
CHEMBL92387 1 8.25
CHEMBL327819 METHAMILANE 1 8.19
CHEMBL12587 MUSCARINE 1 7.92
CHEMBL14 CARBACHOL 4.0 1 7.78
CHEMBL7303 ARECOLINE -1.0 1 7.70
CHEMBL88578 1 7.35
CHEMBL313227 1 7.27
CHEMBL550 PILOCARPINE 4.0 1 7.10
CHEMBL37132 1 7.00
CHEMBL315191 1 6.50
CHEMBL316309 1 6.48
CHEMBL91776 1 6.37
CHEMBL92429 1 6.35
CHEMBL327363 1 6.15
CHEMBL329000 1 6.10
CHEMBL90409 1 6.07
CHEMBL313046 PILOSININE 1 5.54
CHEMBL90532 1 5.34
CHEMBL92257 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL7634 OXOTREMORINE IC50 = 2.8 nM 8.55
CHEMBL92387 IC50 = 5.6 nM 8.25
CHEMBL327819 METHAMILANE IC50 = 6.4 nM 8.19
CHEMBL12587 MUSCARINE IC50 = 12.0 nM 7.92
CHEMBL14 CARBACHOL IC50 = 16.5 nM 7.78
CHEMBL7303 ARECOLINE IC50 = 20.0 nM 7.70
CHEMBL88578 IC50 = 45.0 nM 7.35
CHEMBL313227 IC50 = 54.0 nM 7.27
CHEMBL550 PILOCARPINE IC50 = 80.0 nM 7.10
CHEMBL37132 IC50 = 100.0 nM 7.00
CHEMBL315191 IC50 = 320.0 nM 6.50
CHEMBL316309 IC50 = 330.0 nM 6.48
CHEMBL91776 IC50 = 430.0 nM 6.37
CHEMBL92429 IC50 = 445.0 nM 6.35
CHEMBL327363 IC50 = 705.0 nM 6.15
CHEMBL329000 IC50 = 800.0 nM 6.10
CHEMBL90409 IC50 = 845.0 nM 6.07
CHEMBL313046 PILOSININE IC50 = 2890.0 nM 5.54
CHEMBL90532 IC50 = 4600.0 nM 5.34
CHEMBL92257 IC50 = 10000.0 nM 5.00