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Assay Detail

CHEMBL744770

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity towards M1 muscarinic receptor expressed in A9 L cells by displacing [3H](R)-QNB radioligand.
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type L929
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M1 (CHEMBL216)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and biological characterization of bivalent 1-methyl-1,2,5,6-tetrahydropyridyl-1,2,5-thiadiazole derivatives as selective muscarinic agonists.
Rajeswaran WG, Cao Y, Huang XP, Wroblewski ME, Colclough T, Lee S, Liu F, Nagy PI, Ellis J, Levine BA, Nocka KH, Messer WS.
J Med Chem (2001) 44 : 4563 -4576
PubMed 11741475 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 21 8.59 9.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL335542 1 9.92
CHEMBL141452 1 9.72
CHEMBL143468 1 9.64
CHEMBL143434 1 9.49
CHEMBL143466 1 9.24
CHEMBL142126 1 9.21
CHEMBL139603 1 9.16
CHEMBL143469 1 9.08
CHEMBL341759 1 9.06
CHEMBL142184 1 9.03
CHEMBL337131 1 8.92
CHEMBL140142 1 8.82
CHEMBL142538 1 8.80
CHEMBL139887 1 8.72
CHEMBL140789 1 8.30
CHEMBL140985 1 8.12
CHEMBL140746 1 7.85
CHEMBL336933 1 7.77
CHEMBL343055 1 7.70
CHEMBL21536 XANOMELINE 4.0 1 7.09
CHEMBL14 CARBACHOL 4.0 1 4.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL335542 Ki = 0.12 nM 9.92
CHEMBL141452 Ki = 0.19 nM 9.72
CHEMBL143468 Ki = 0.23 nM 9.64
CHEMBL143434 Ki = 0.32 nM 9.49
CHEMBL143466 Ki = 0.57 nM 9.24
CHEMBL142126 Ki = 0.61 nM 9.21
CHEMBL139603 Ki = 0.69 nM 9.16
CHEMBL143469 Ki = 0.84 nM 9.08
CHEMBL341759 Ki = 0.87 nM 9.06
CHEMBL142184 Ki = 0.93 nM 9.03
CHEMBL337131 Ki = 1.2 nM 8.92
CHEMBL140142 Ki = 1.5 nM 8.82
CHEMBL142538 Ki = 1.6 nM 8.80
CHEMBL139887 Ki = 1.9 nM 8.72
CHEMBL140789 Ki = 5.0 nM 8.30
CHEMBL140985 Ki = 7.6 nM 8.12
CHEMBL140746 Ki = 14.0 nM 7.85
CHEMBL336933 Ki = 17.0 nM 7.77
CHEMBL343055 Ki = 20.0 nM 7.70
CHEMBL21536 XANOMELINE Ki = 82.0 nM 7.09
CHEMBL14 CARBACHOL Ki = 20000.0 nM 4.70