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Assay Detail

CHEMBL615153

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity of aminoglycoside to 16S ribosomal RNA A-site in Escherichia coli
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 3 — Cell-line / Tissue
Curated By Expert

Publication

Which aminoglycoside ring is most important for binding? A hydropathic analysis of gentamicin, paromomycin, and analogues.
Cashman DJ, Rife JP, Kellogg GE.
Bioorg Med Chem Lett (2001) 11 : 119 -122
PubMed 11206440 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 14 5.69 7.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL184618 FRAMYCETIN 3.0 1 7.72
CHEMBL370143 PAROMOMYCIN 4.0 1 6.70
CHEMBL606550 1 6.58
CHEMBL1159506 1 6.16
CHEMBL176 BEKANAMYCIN 3.0 1 5.85
CHEMBL1747 TOBRAMYCIN 4.0 1 5.82
CHEMBL1159782 1 5.77
CHEMBL301721 GENTAMICIN C1A 1 5.77
CHEMBL427409 NEAMINE 1 5.11
CHEMBL223520 KANAMYCIN A 1.0 1 4.75
CHEMBL221572 RIBOSTAMYCIN 2.0 1 4.60
CHEMBL605012 BUTIROSIN B 1 4.57
CHEMBL606551 1 4.55
CHEMBL606552 PAROMAMINE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL184618 FRAMYCETIN Kd = 19.0 nM 7.72
CHEMBL370143 PAROMOMYCIN Kd = 200.0 nM 6.70
CHEMBL606550 Kd = 260.0 nM 6.58
CHEMBL1159506 Kd = 700.0 nM 6.16
CHEMBL176 BEKANAMYCIN Kd = 1400.0 nM 5.85
CHEMBL1747 TOBRAMYCIN Kd = 1500.0 nM 5.82
CHEMBL1159782 Kd = 1700.0 nM 5.77
CHEMBL301721 GENTAMICIN C1A Kd = 1700.0 nM 5.77
CHEMBL427409 NEAMINE Kd = 7800.0 nM 5.11
CHEMBL223520 KANAMYCIN A Kd = 18000.0 nM 4.75
CHEMBL221572 RIBOSTAMYCIN Kd = 25000.0 nM 4.60
CHEMBL605012 BUTIROSIN B Kd = 27000.0 nM 4.57
CHEMBL606551 Kd = 28000.0 nM 4.55
CHEMBL606552 PAROMAMINE Kd < 100000.0 nM -